cNPAS2 induced β cell dysfunction by regulating KANK1 expression in type 2 diabetes.

Autor:	Yin YB; Department of General Surgery, The First Affiliated Hospital of Jiamusi University, Jiamusi 154000, Heilongjiang Province, China., Ji W; Department of Anesthesiology, Yantai Affiliated Hospital of Binzhou Medical University, Yantai 264199, Shandong Province, China., Liu YL; Operating Room, The First Affiliated Hospital of Jiamusi University, Jiamusi 154000, Heilongjiang Province, China., Gao QH; Department of Anesthesiology, Huazhong University of Science and Technology Union Jiangbei Hospital, Wuhan 430100, Hubei Province, China., He DD; Department of Endocrinology, The First Affiliated Hospital of Jiamusi University, Jiamusi 154000, Heilongjiang Province, China., Xu SL; Department of General Surgery, The First Affiliated Hospital of Jiamusi University, Jiamusi 154000, Heilongjiang Province, China., Fan JX; Department of Endocrinology, The First Affiliated Hospital of Jiamusi University, Jiamusi 154000, Heilongjiang Province, China., Zhang LH; Department of General Surgery, The First Affiliated Hospital of Jiamusi University, Jiamusi 154000, Heilongjiang Province, China. zhanglihai@jmsu.edu.cn.
Jazyk:	angličtina
Zdroj:	World journal of diabetes [World J Diabetes] 2024 Sep 15; Vol. 15 (9), pp. 1932-1941.
DOI:	10.4239/wjd.v15.i9.1932
Abstrakt:	Background: Diabetes mellitus type 2 (T2DM) is formed by defective insulin secretion with the addition of peripheral tissue resistance of insulin action. It has been affecting over 400 million people all over the world. Aim: To explore the pathogenesis of T2DM and to develop and implement new prevention and treatment strategies for T2DM. Methods: Receiver operating characteristic (ROC) curve analysis was used to conduct diagnostic markers. The expression level of genes was determined by reverse transcription-PCR as well as Western blot. Cell proliferation assays were performed by cell counting kit-8 (CCK-8) tests. At last, T2DM mice underwent Roux-en-Y gastric bypass surgery. Results: We found that NPAS2 was significantly up-regulated in islet β cell apoptosis of T2DM. The ROC curve revealed that NPAS2 was capable of accurately diagnosing T2DM. NPAS2 overexpression did increase the level of KANK1 . In addition, the CCK-8 test revealed knocking down NPAS2 and KANK1 increased the proliferation of MIN6 cells. At last, we found that gastric bypass may treat type 2 diabetes by down-regulating NPAS2 and KANK1 . Conclusion: This study demonstrated that NPAS2 induced β cell dysfunction by regulating KANK1 expression in type 2 diabetes, and it may be an underlying therapy target of T2DM. Competing Interests: Conflict-of-interest statement: The authors have no relevant financial or non-financial interests to disclose. (©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.)
Databáze:	MEDLINE
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