Analysis of B cell proliferation in response to in vitro stimulation in patients with CVID.

Autor:	Guadalupe VM; Posgrado en Ciencias Biológicas UNAM, Ciudad Universitaria, México., Abigail J SS; Departamento de Atención de la Salud, Universidad Autónoma Metropolitana, Unidad Xochimilco, Ciudad de México, México., Patricia OR; Servicio de Alergia e Inmunología Clínica, Hospital de Especialidades del Centro Médico Siglo XXI, IMSS., Andrea HD; Servicio de Alergia e Inmunología Clínica, Hospital de Especialidades del Centro Médico Siglo XXI, IMSS., Selma SM; Servicio de Inmunología y Alergia, Instituto Nacional de Pediatría SSA, Ciudad de México, México., Marco A YN; Servicio de Inmunología y Alergia, Instituto Nacional de Pediatría SSA, Ciudad de México, México., Carmen ZM; Servicio de Alergia, Hospital Universitario, Monterrey, Nuevo León., Sara E EP; Laboratorio en Inmunodeficiencias, Instituto Nacional de Pediatría SSA, Ciudad de México, México., Berrón-Ruiz L; Laboratorio en Inmunodeficiencias, Instituto Nacional de Pediatría SSA, Ciudad de México, México; lberronruiz@gmail.com.
Jazyk:	angličtina
Zdroj:	Allergologia et immunopathologia [Allergol Immunopathol (Madr)] 2024 Sep 01; Vol. 52 (5), pp. 94-102. Date of Electronic Publication: 2024 Sep 01 (Print Publication: 2024).
DOI:	10.15586/aei.v52i5.1106
Abstrakt:	Background: Common variable immunodeficiency (CVID) is a heterogeneous disorder characterized by defective antibody production and impaired differentiation of B cells. B cell proliferation is an essential step for antibody synthesis. Depending on the nature of the stimulus, their response may be either T-cell-dependent or T-cell-independent. Methods: We studied 23 CVID patients and 14 healthy donors (HD). The patients were categorized based on their percentage of memory B cells. In addition to standard immunophenotyping of circulating human B and T cell subsets, an in vitro CFSE dilution assay was used to assess the proliferative capacity of B cells and to compare the activation of the T cell-dependent and T cell-independent response among the patients. Results: Patients with a reduction in memory B cells exhibited an increase in follicular T cells (Tfh) and showed low proliferation in response to PKW, CpG, and SAC stimuli (Condition II) ( p = 0.0073). In contrast, patients with a normal percentage of memory B cells showed a high expression of IL-21R and low proliferation in response to CPG (Condition III); IL-21, CD40L, and anti-IgM (Condition IV) stimuli ( p = 0.0163 and p = 0.0475, respectively). Conclusion: Defective proliferation in patients depends on the type of stimulus used and the phenotypic characteristics of the patients. Further studies are necessary to understand the disease mechanisms, which may guide us toward identifying genetic defects associated with CVID.
Databáze:	MEDLINE
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