Effects of exposure to 17α-methyltestosterone on hepatic lipid metabolism in Gobiocypris rarus.

Autor: Li T; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China., Xiong Z; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China., Rong W; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China., Yang Q; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China., Chen Y; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China., Zhao H; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China., Liu Q; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China., Song J; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China., Wang W; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China., Liu Y; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China., Wang X; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China. Electronic address: xianzong_wang@126.com., Liu S; College of Animal Science, Shanxi Agricultural University, Jinzhong 030801, China; Shanxi Key Laboratory of Animal Genetics Resource Utilization and Breeding, Jinzhong 030801, China. Electronic address: shmily8316@126.com.
Jazyk: angličtina
Zdroj: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP [Comp Biochem Physiol C Toxicol Pharmacol] 2025 Jan; Vol. 287, pp. 110041. Date of Electronic Publication: 2024 Sep 14.
DOI: 10.1016/j.cbpc.2024.110041
Abstrakt: This study aimed to investigate the effects of 17α-Methyltestosterone (MT) on hepatic lipid metabolism in Gobiocypris rarus. G. rarus was exposed to varying concentrations of MT (0, 25, 50, and 100 ng/L) for durations of 7, 14, and 21 d. Biochemical and transcriptomic analyses were conducted using methods, such as ELISA, RT-qPCR, Western Blotting, and RNA-seq, to decipher the key signals and molecular mechanisms triggered by MT in vivo. The results revealed that MT induced hepatomegaly in G. rarus and markedly increased the hepatic steatosis index (HSI). After 14 d of exposure, significant increase in PPARγ mRNA expression was observed, whereas after 21 d, PPARα mRNA expression was significantly reduced. The expression pattern of SREBP1C mRNA initially decreased before increasing, mirroring the trend observed for SREBP1C protein expression. Furthermore, MT increased the levels of key lipid synthesis enzymes, including HSL, CPT1, GPAT, and FAS, thereby fostering lipid accumulation. RNA-seq analysis revealed that MT modulated hepatic bile acid metabolism via the PPAR pathway, consequently influencing cholesterol and lipid metabolism. Considering the differential metabolic pathways of MT across genders, it is postulated that MT may undergo aromatization to estrogen within G. rarus, thereby exerting estrogenic effects. These findings provide crucial experimental insights into the detrimental effects of MT in aquatic settings, underscoring its implications for safeguarding aquatic organisms and human health.
Competing Interests: Declaration of competing interest The authors declare that there are no conflicts of interest.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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