Relationship between systemic biomarker of lipid peroxidation 4-hydroxynonenal and lipidomic profile of morbidly obese patients undergoing bariatric surgery.

Autor: Halasz M; Laboratory for Oxidative Stress, Division of Molecular Medicine, Ruder Boskovic Institute, 10000, Zagreb, Croatia., Łuczaj W; Department of Analytical Chemistry, Medical University of Białystok, 15-222 Białystok, Poland., Jarocka-Karpowicz I; Department of Analytical Chemistry, Medical University of Białystok, 15-222 Białystok, Poland., Stasiewicz A; Department of Analytical Chemistry, Medical University of Białystok, 15-222 Białystok, Poland., Soldo AM; Internal Medicine Clinic, University Hospital 'Sveti Duh', Zagreb, Croatia., Soldo I; Surgery Clinic, University Hospital 'Sveti Duh', Zagreb, Croatia., Pajtak A; Department of Abdominal Surgery, General Hospital Varazdin, Varazdin, Croatia., Senčar M; Department of Abdominal Surgery, General Hospital Varazdin, Varazdin, Croatia., Grgić T; Surgery Clinic, University Hospital 'Sveti Duh', Zagreb, Croatia., Kolak T; Department of Abdominal Surgery, University Hospital Dubrava, Zagreb, Croatia., Žarković N; Laboratory for Oxidative Stress, Division of Molecular Medicine, Ruder Boskovic Institute, 10000, Zagreb, Croatia., Skrzydlewska E; Department of Analytical Chemistry, Medical University of Białystok, 15-222 Białystok, Poland. Electronic address: elzbieta.skrzydlewska@umb.edu.pl., Jaganjac M; Laboratory for Oxidative Stress, Division of Molecular Medicine, Ruder Boskovic Institute, 10000, Zagreb, Croatia. Electronic address: morana.jaganjac@irb.hr.
Jazyk: angličtina
Zdroj: Free radical biology & medicine [Free Radic Biol Med] 2024 Nov 01; Vol. 224, pp. 564-573. Date of Electronic Publication: 2024 Sep 13.
DOI: 10.1016/j.freeradbiomed.2024.09.018
Abstrakt: Obesity is characterized by fat accumulation, impaired metabolism and oxidative stress, frequently associated with lipid peroxidation and generation of bioactive 4-hydroxynonenal (4-HNE). This study aimed to evaluate the impact of bariatric surgery-induced weight loss on lipid peroxidation and associated perturbations in lipid profile. Plasma samples of twenty obese individuals before and 6 months after bariatric surgery were collected in addition to samples of ten healthy controls. HILIC-LC-MS/MS platform was used to characterize phospholipid profile, while lipid peroxidation markers 15-F2t-IsoP, 10-F4t-NeuroP and reactive aldehyde 4-HNE were quantified by RP-LC-MS/MS and GC-MS, respectively. Six months post-surgery lipid peroxidation markers decreased significantly and the BMI of morbidly obese patients decreased by 13 on average. Lipidomics analysis, identified 117 phospholipid species from seven classes, and showed obesity-associated lipidome perturbations, particularly in ether-linked phosphatidylethanolamines (PEo). A total of 45 lipid species were found to be significantly altered with obesity, while 10 lipid species correlated with lipid peroxidation markers. Sample pairwise analyses indicated an interesting link between 4-HNE and the amount of two PEos, PEo (38:2) and PEo (36:2). The results indicate that weight loss-induced improvement of redox homeostasis together with changes in lipid metabolites may serve as markers of metabolic improvement. However, further studies are needed to understand the role of obesity-induced oxidative stress on ether lipid biosynthesis and lipidome perturbations, as well as the impact of bariatric surgery on metabolic improvement.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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