Detection of egg white allergy in children by specific IgE microarray chemiluminescence immunoassay.

Autor: Zhang RW; Department of Medical Laboratory, Affiliated Children's Hospital of Jiangnan University, Qingyang Road 299, Wuxi, Jiangsu, 214023, China., Yuan DD; Department of Medical Laboratory, Affiliated Children's Hospital of Jiangnan University, Qingyang Road 299, Wuxi, Jiangsu, 214023, China., Yang X; Department of Medical Laboratory, Affiliated Children's Hospital of Jiangnan University, Qingyang Road 299, Wuxi, Jiangsu, 214023, China., Yang YB; Department of Medical Laboratory, Affiliated Children's Hospital of Jiangnan University, Qingyang Road 299, Wuxi, Jiangsu, 214023, China., Li FP; Department of Medical Laboratory, Affiliated Children's Hospital of Jiangnan University, Qingyang Road 299, Wuxi, Jiangsu, 214023, China., Chen XY; Department of Medical Laboratory, Affiliated Children's Hospital of Jiangnan University, Qingyang Road 299, Wuxi, Jiangsu, 214023, China., Wang K; Department of Medical Laboratory, Affiliated Children's Hospital of Jiangnan University, Qingyang Road 299, Wuxi, Jiangsu, 214023, China. Electronic address: hyxj0309@163.com., Liu J; Department of Respiratory and Critical Care Medicine, Affiliated Wuxi People's Hospital of Nanjing Medical University, Wuxi, Jiangsu, 214023, China. Electronic address: liujie730125@163.com., Yu LN; Jiangsu Sunlant Bioengineering Co., Ltd, Wuxi, Jiangsu, 214038, China. Electronic address: 369768871@qq.com., Hu ZG; Department of Medical Laboratory, Affiliated Children's Hospital of Jiangnan University, Qingyang Road 299, Wuxi, Jiangsu, 214023, China. Electronic address: jswxhzg@163.com.
Jazyk: angličtina
Zdroj: Clinica chimica acta; international journal of clinical chemistry [Clin Chim Acta] 2025 Jan 15; Vol. 565, pp. 119966. Date of Electronic Publication: 2024 Sep 13.
DOI: 10.1016/j.cca.2024.119966
Abstrakt: Background: Allergen testing has emerged as a pivotal component in prevention and treatment strategies for allergic diseases among children and the utilization of specific IgE (sIgE) through a fully automated chemiluminescent microarray immunoassay (CLMIA) has emerged as a promising trend in the simultaneous detection of multiple allergenic components of children.
Methods: The accuracy and reliability of CLMIA were verified using children's serum samples that concentrated on allergens. the allergens. The clinical diagnostic practicability of CLMIA was assessed through comprehensive evaluations including measurements of the limit of detection (LOD), intra-batch, and inter-batch precision, linearity analysis, the cross-contamination rate, and the concordance rate with the Phadia system.
Results: After the optimization process of CLMIA, the LODs for allergens were calculated to be below 0.01 kU/L, demonstrating the high sensitivity of CLMIA. All components exhibited good linearity within the range of 0.1-100.0 kU/L and the coefficient of determinations (R 2  > 0.99). The data of intra-batch precision (<10 %) and inter-batch data (<15 %) illustrated the high reproducibility of CLMIA. The cross-contamination rates for allergens (<0.5 %) showed the high accuracy of CLMIA without interfering. The positive concordance rate between CLMIA and the Phadia system exceeds 90 % with a good negative concordance rate (>85 %) and the Kappa coefficients (>0.8), suggesting the close alignment of CLMIA and the Phadia system and showing the satisfactory clinical potential of CLMIA in children's allergy disease.
Conclusions: The application of CLMIA has been promising in allergen testing, especially for detecting multiple allergenic components in children.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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