Analysis of miR-483-3p and miR-630 expression profile in pediatric adrenocortical tumors and the effect of their modulation on adrenal tumorigenesis in vitro.

Autor: Corrêa CAP; Department of Genetics - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., Andrade AF; Department of Human Genetics, McGrill University, Montreal, Canada., Veronez LC; Department of Pediatrics - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., da Silva KR; Department of Genetics - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., Baroni M; Department of Genetics - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., Suazo VK; Department of Pediatrics - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., de Paula Gomes Queiroz R; Department of Pediatrics - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., Lira RCP; Department of Genetics - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil; Division of General Pathology, Federal University of Triângulo Mineiro, Campus I, Uberaba, MG, 38025-200, Brazil., Chagas PS; Department of Genetics - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., Brandalise SR; Boldrini Children's Center, Campinas, Brazil., Yunes JA; Boldrini Children's Center, Campinas, Brazil., Molina CAF; Department of Surgery - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., Antonini SRR; Department of Pediatrics - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., Valera ET; Department of Pediatrics - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., Tone LG; Department of Genetics - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil; Department of Pediatrics - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil., Scrideli CA; Department of Genetics - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil; Department of Pediatrics - Ribeirão Preto Medical School, University of São Paulo, Ribeirão Preto, Brazil; National Science and Technology Institute for Children's Cancer Biology and Pediatric Oncology - INCT BioOncoPed, Brazil. Electronic address: scrideli@fmrp.usp.br.
Jazyk: angličtina
Zdroj: Molecular and cellular endocrinology [Mol Cell Endocrinol] 2024 Dec 01; Vol. 594, pp. 112371. Date of Electronic Publication: 2024 Sep 14.
DOI: 10.1016/j.mce.2024.112371
Abstrakt: Pediatric adrenocortical tumors (ACT) are rare aggressive neoplasms with heterogeneous prognosis. MicroRNA (miRNA) signatures have been associated with cancer diagnosis, treatment response, and outcomes of several types of cancer. However, the role played by miRNAs in pediatric ACT has been poorly explored. In this study, we have evaluated the expression of miR-483-3p and miR-630 in 67 pediatric ACT and 19 non-neoplastic adrenal samples, the effects of the modulations of these miRNAs, and their relationship with the TGF-β pathway in the H295R and H295A cell lines. Deregulation of both miRNAs was related to survival and disease advanced stages and hence to patients' prognosis. Moreover, modified miR-483-3p and miR-630 in vitro expression decreased cell viability and colony formation capacity, changed how some genes of the TGF-β pathway, such as TGFBR1, TGFBR2, and SMAD7, are expressed, and altered Smad3, pSmad3, Smad 2/3, N-cadherin, and Vimentin protein expression. Besides that, when inhibition of the TGF-β pathway was combined with miR-630 overexpression or miR-483-3p silencing, cell viability and colony formation capacity decreased, and protein expression in the TGF-β pathway changed. Together, the data indicate that both miRNAs participate in the TGF-β pathway and are therefore potential markers for predicting the prognosis of patients with pediatric ACT.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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