Evaluation of the effect of taurine on the matrix metalloproteinase-9 and the expression changes of miRNA-21 and miRNA-146a in SH-SY5Y cell line.
| Autor: | Al-Attabi A; College of Medicine, 294060 University of Thi-Qar , Al-Nasiriya, Iraq., Mukhlif BA; Department of Medical Laboratories Techniques, College of Health and Medical Technology, University of Al Maarif, Al Anbar, Iraq., Al-Shami KR; Department of Forensic Sciences, College of Science, National University of Science and Technology, Dhi Qar, Iraq., Merza MS; Prosthetic Dental Techniques Department, Al-Mustaqbal, University College, Hillah, Babylon, Iraq., Alkubaisy SA; Desert Studies Center, University of Anbar, Al-Anbar, Iraq., Abdulhadi MA; Department of Biochemistry, College of Medicine, University of Anbar, Ramadi City, Al-Anbar Governorate, Iraq. |
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| Jazyk: | angličtina |
| Zdroj: | Hormone molecular biology and clinical investigation [Horm Mol Biol Clin Investig] 2024 Sep 16; Vol. 45 (4), pp. 165-170. Date of Electronic Publication: 2024 Sep 16 (Print Publication: 2024). |
| DOI: | 10.1515/hmbci-2024-0005 |
| Abstrakt: | Objectives: Alzheimer's disease (AD), a brain disorder, is the leading cause of dementia among older adults. Taurine, an amino acid abundantly present in the brain, and shows potential neuroprotective properties. Therefore, we investigated the effects of taurine on Matrix Metalloproteinase-9 (MMP-9) levels and the expression changes of miRNA-21 and miRNA-146a in the SH-SY5Y cell line. Methods: Taurine's impact on the SH-SY5Y cell line was evaluated via the 2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. MMP-9 levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit, while the expression of miRNA-21 and miRNA-146a genes was assessed through Real-Time PCR analysis. Results: The MTT assay revealed no toxic effects on SH-SY5Y cells with increasing concentrations of taurine. Analysis of gene expression indicated a rise in miRNA-21 expression and a decline in miRNA-146 expression with increasing taurine concentration, with the most notable change observed at 1 mg/mL taurine (p<0.001). ELISA results demonstrated a significant increase in MMP-9 levels in the SH-SY5Y cell line treated with 1 mg/mL taurine compared to the untreated group (p<0.001). Conclusions: Our study revealed that taurine can alter the expression of miRNA-146a and miRNA-21. In conclusion, taurine therapy presents promising therapeutic avenues for treating AD or mitigating severe symptoms. Nonetheless, further research is necessary to comprehensively grasp the precise mechanisms at play. (© 2024 Walter de Gruyter GmbH, Berlin/Boston.) |
| Databáze: | MEDLINE |
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