Improved functionality of hepatic spheroids cultured in acoustic levitation compared to existing 2D and 3D models.

Autor: Rabiet L; Laboratoire Physique et Mécanique des Milieux Hétérogènes (PMMH), CNRS, ESPCI, 7 Quai Saint-Bernard, 75005, Paris, France. lucile.rabiet@gmail.com.; Inserm U976, CIC-BT CBT501, AP-HP, Université Paris-Cité, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75010, Paris, France. lucile.rabiet@gmail.com., Jeger-Madiot N; Laboratoire Physique et Mécanique des Milieux Hétérogènes (PMMH), CNRS, ESPCI, 7 Quai Saint-Bernard, 75005, Paris, France., García DR; Laboratoire Physique et Mécanique des Milieux Hétérogènes (PMMH), CNRS, ESPCI, 7 Quai Saint-Bernard, 75005, Paris, France., Tosca L; Service Histologie Embryologie Cytogénomique, Hôpital Antoine Béclère, 157 Rue de la Porte de Trivaux, 92140, Clamart, France., Tachdjian G; Service Histologie Embryologie Cytogénomique, Hôpital Antoine Béclère, 157 Rue de la Porte de Trivaux, 92140, Clamart, France., Kellouche S; Laboratoire ERRMECe, Maison Internationale de la Recherche, CY Cergy Paris Université, 1 rue Descartes, 95000, Neuville-sur-Oise, France., Agniel R; Laboratoire ERRMECe, Maison Internationale de la Recherche, CY Cergy Paris Université, 1 rue Descartes, 95000, Neuville-sur-Oise, France., Larghero J; Inserm U976, CIC-BT CBT501, AP-HP, Université Paris-Cité, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75010, Paris, France., Aider JL; Laboratoire Physique et Mécanique des Milieux Hétérogènes (PMMH), CNRS, ESPCI, 7 Quai Saint-Bernard, 75005, Paris, France. jean-luc.aider@espci.psl.eu., Arakelian L; Inserm U976, CIC-BT CBT501, AP-HP, Université Paris-Cité, Hôpital Saint-Louis, 1 avenue Claude Vellefaux, 75010, Paris, France. lousineh.arakelian@aphp.fr.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Sep 14; Vol. 14 (1), pp. 21528. Date of Electronic Publication: 2024 Sep 14.
DOI: 10.1038/s41598-024-72059-x
Abstrakt: Hepatic spheroids are of high interest in basic research, drug discovery and cell therapy. Existing methods for spheroid culture present advantages and drawbacks. An alternative technology is explored: the hepatic spheroid formation and culture in an acoustofluidic chip, using HepaRG cell line. Spheroid formation and morphology, cell viability, genetic stability, and hepatic functions are analyzed after 6 days of culture in acoustic levitation. They are compared to 2D culture and non-levitated 3D cultures. Sizes of the 25 spheroids created in a single acoustofluidic microphysiological system are homogeneous. The acoustic parameters in our system do not induce cell mortality nor DNA damage. Spheroids are cohesive and dense. From a functional point of view, hepatic spheroids obtained by acoustic levitation exhibit polarity markers, secrete albumin and express hepatic genes at higher levels compared to 2D and low attachment 3D cultures. In conclusion, this microphysiological system proves not only to be suitable for long-term culture of hepatic spheroids, but also to favor differentiation and functionality within 6 days of culture.
(© 2024. The Author(s).)
Databáze: MEDLINE
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