Evaluating the Impact of Ultrasonic Irrigation on Bacterial Levels and Activity Following Chemomechanical Procedures.

Autor:	Nogales CG; Department of Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil., Cazares RXR; Department of Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil; Discipline of Endodontics, School of Dentistry, Central University of Ecuador, Quito, Ecuador., Nardello LCL; Department of Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil., Mayer MPA; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil., Gavini G; Department of Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil., Zehnder M; Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, University of Zurich, Zürich, Switzerland., Pinheiro ET; Department of Dentistry, School of Dentistry, University of São Paulo, São Paulo, Brazil. Electronic address: erickapinheiro@usp.br.
Jazyk:	angličtina
Zdroj:	Journal of endodontics [J Endod] 2024 Sep 12. Date of Electronic Publication: 2024 Sep 12.
DOI:	10.1016/j.joen.2024.09.001
Abstrakt:	Introduction: This single-arm interventional trial aimed to investigate the efficacy of ultrasonic irrigation as a supplementary disinfection approach after chemomechanical procedures (CMP) using molecular techniques based on ribosomal RNA (rRNA) and rRNA genes (referred to as DNA). Methods: Samples were collected from 35 single-rooted teeth with radiographic evidence of apical periodontitis. Samples were taken after gaining root canal access (S1), (S2), and ultrasonic irrigation (S3). DNA-targeted quantitative polymerase chain reaction using universal primers was used to estimate total bacterial levels, while rRNA-targeted quantitative polymerase chain reaction was used to assess bacterial activity. Ratios between rRNA and DNA levels were calculated to search for active bacteria in the samples (rRNA/DNA ≥ 1). Wilcoxon matched-pairs signed-rank test was used to compare the differences in DNA levels between samples and DNA and rRNA levels within samples (P < .05). Results: DNA-based methods revealed a significant decrease in bacterial levels from S1 to S2 and S2 to S3 (both P < .05). Notably, 11 out of 35 (31.4%) root canals did not harbor bacterial DNA after CMP, whereas ultrasonic activation increased DNA-negative samples to 17 (48.6%). However, all DNA-positive samples were also positive for rRNA, with significantly higher rRNA than DNA levels (P < .05), indicating bacterial activity at the sampling time. Conclusions: Ultrasonic irrigation improved the disinfection of root canals after CMP by reducing bacterial levels. However, persisting bacteria remained active in the root canals after CMP and ultrasonic irrigation. (Copyright © 2024 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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