Portable label-free electrochemical aptasensor for sensitive detection of paraquat herbicide mediated by oxygen reduction reaction.

Autor: Phopin K; Center for Research Innovation and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Nakhon Pathom, 73170, Thailand; Department of Clinical Microbiology and Applied Technology, Faculty of Medical Technology, Bangkok, 10700, Thailand., Ruankham W; Center for Research Innovation and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Nakhon Pathom, 73170, Thailand., Dave J; Center for Research Innovation and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Nakhon Pathom, 73170, Thailand., Rukkhapiban P; Center for Research Innovation and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Nakhon Pathom, 73170, Thailand., Nuttavuttisit C; Center for Research Innovation and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Nakhon Pathom, 73170, Thailand., Hongoeb J; Center for Research Innovation and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Nakhon Pathom, 73170, Thailand., Zine N; Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon-1, Villeurbanne, 69100, France., Errachid A; Institut des Sciences Analytiques (ISA), Université Claude Bernard Lyon-1, Villeurbanne, 69100, France., Tantimongcolwat T; Center for Research Innovation and Biomedical Informatics, Faculty of Medical Technology, Mahidol University, Nakhon Pathom, 73170, Thailand. Electronic address: tanawut.tan@mahidol.ac.th.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2025 Jan 01; Vol. 281, pp. 126841. Date of Electronic Publication: 2024 Sep 10.
DOI: 10.1016/j.talanta.2024.126841
Abstrakt: Paraquat (PQ) is a highly toxic herbicide that has been prohibited in almost 70 countries, but remains in use worldwide. Thus, routine on-site PQ monitoring is a key mechanism to ensure safety and efficiently enforce regulations. Herein, a label-free portable electrochemical aptasensor for the detection of PQ was developed by utilizing aptamer designed to specifically recognize PQ. The aptasensor employs square-wave voltammetry (SWV) to quantify PQ binding on the aptamer-functionalized electrode surface by tracking the downstream oxygen reduction reaction. It provided a detection range spanning from 0.01 to 100.0 μg mL - 1 PQ with a limit of detection (LOD) of 8.9 ng mL - 1 . Validation against spiked tap water, pomegranate juice, and orange juice revealed recovery rate performances of 75 %-130 %. The aptasensor demonstrates promising feasibility for PQ detection in real-world applications, offering remarkable portability and operational simplicity. Notably, it can operate without supplementary redox agents, requiring only sample incubation and subsequent washing steps.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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