CRISPR/Cas9-mediated generation of AP-1 activity reporter cell line in human embryonic stem cell (WAe007-A-5).

Autor:	Guo X; Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou 215123, Jiangsu, PR China; Suzhou Medical College, Soochow University, Suzhou 215123, Jiangsu, PR China; Department of Vascular Surgery, Suqian First Hospital, Suqian 223800, Jiangsu, PR China., Zhang M; Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou 215123, Jiangsu, PR China; Suzhou Medical College, Soochow University, Suzhou 215123, Jiangsu, PR China., Xu Z; Suzhou Medical College, Soochow University, Suzhou 215123, Jiangsu, PR China; School of Biology & Basic Medical Sciences, Suzhou Medical College, Soochow University, Suzhou 215123, Jiangsu, PR China., Yu Y; Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou 215123, Jiangsu, PR China; Suzhou Medical College, Soochow University, Suzhou 215123, Jiangsu, PR China. Electronic address: yyu@suda.edu.cn., Shen Z; Department of Cardiovascular Surgery of the First Affiliated Hospital & Institute for Cardiovascular Science, Soochow University, Suzhou 215123, Jiangsu, PR China; Suzhou Medical College, Soochow University, Suzhou 215123, Jiangsu, PR China. Electronic address: uuzyshen@aliyun.com.
Jazyk:	angličtina
Zdroj:	Stem cell research [Stem Cell Res] 2024 Dec; Vol. 81, pp. 103557. Date of Electronic Publication: 2024 Sep 13.
DOI:	10.1016/j.scr.2024.103557
Abstrakt:	Activator protein 1 (AP-1) is involved in cell fate determination and function. To monitor the AP-1 activity, we cloned a AP-1 binding sites fragment into the upstream of minimal TATA-box promoter, then a luciferase-GFP reporter (LuciGFP) was designated to the downstream of the promoter. With CRISPR/Cas9, the AP-1-LuciGFP reporter was introduced into AAVS1 locus, a safe harbor for gene editing. Thus, this AP-1-LuciGFP reporter cell line could be subjected to monitor the AP-1 activity during the cell differentiation, cell fate transition and disease modeling. Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze:	MEDLINE
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