Genome-wide association study and molecular marker development for susceptibility to Gibberella ear rot in maize.

Autor: Zhou G; Jiangsu Yanjiang Institute of Agricultural Sciences, Nantong, 226012, China. gfzhou88@jaas.ac.cn.; State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing, 210095, China. gfzhou88@jaas.ac.cn.; Collaborative Innovation Center for Modern Crop Production Co-sponsored by Province and Ministry/College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China. gfzhou88@jaas.ac.cn., Ma L; Jiangsu Yanjiang Institute of Agricultural Sciences, Nantong, 226012, China.; State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing, 210095, China.; Collaborative Innovation Center for Modern Crop Production Co-sponsored by Province and Ministry/College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China., Zhao C; State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing, 210095, China.; Collaborative Innovation Center for Modern Crop Production Co-sponsored by Province and Ministry/College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China., Xie F; State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing, 210095, China.; Collaborative Innovation Center for Modern Crop Production Co-sponsored by Province and Ministry/College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China., Xu Y; Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, 225009, China., Wang Q; State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing, 210095, China.; Collaborative Innovation Center for Modern Crop Production Co-sponsored by Province and Ministry/College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China., Hao D; Jiangsu Yanjiang Institute of Agricultural Sciences, Nantong, 226012, China., Gao X; State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing, 210095, China. xgao@njau.edu.cn.; Collaborative Innovation Center for Modern Crop Production Co-sponsored by Province and Ministry/College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China. xgao@njau.edu.cn.
Jazyk: angličtina
Zdroj: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik [Theor Appl Genet] 2024 Sep 14; Vol. 137 (10), pp. 222. Date of Electronic Publication: 2024 Sep 14.
DOI: 10.1007/s00122-024-04711-z
Abstrakt: Key Messages: Sixty-nine quantitative trait nucleotides conferring maize resistance to Gibberella ear rot were detected, including eighteen novel loci. Four candidate genes were predicted, and four kompetitive allele-specific PCR markers were developed. Maize Gibberella ear rot (GER), caused by Fusarium graminearum, is one of the most devastating diseases in maize-growing regions worldwide. Enhancing maize cultivar resistance to this disease requires a comprehensive understanding of the genetic basis of resistance to GER. In this study, 334 maize inbred lines were phenotyped for GER resistance in five environments and genotyped using the Affymetrix CGMB56K SNP Array, and a genome-wide association study of resistance to GER was performed using a 3V multi-locus random-SNP-effect mixed linear model. A total of 69 quantitative trait nucleotides (QTNs) conferring resistance to GER were detected, and all of them explained individually less than 10% of the phenotypic variation, suggesting that resistance to GER is controlled by multiple minor-effect genetic loci. A total of 348 genes located around the 200-kb genomic region of these 69 QTNs were identified, and four of them (Zm00001d029648, Zm00001d031449, Zm00001d006397, and Zm00001d053145) were considered candidate genes conferring susceptibility to GER based on gene expression patterns. Moreover, four kompetitive allele-specific PCR markers were developed based on the non-synonymous variation of these four candidate genes and validated in two genetic populations. This study provides useful genetic resources for improving resistance to GER in maize.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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