Sequential Injection Analysis Method for the Determination of Glutathione in Pharmaceuticals.

Autor: Biocic M; Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Rudjera Boskovica 35, 21000 Split, Croatia., Kraljević T; Department of Chemistry, Faculty of Science and Education, University of Mostar, Matice hrvatske bb, 88000 Mostar, Bosnia and Herzegovina., Spassov TG; Faculty of Chemistry and Pharmacy, Sofia University 'St. Kl. Ohridski', 1 James Bourchier Blvd., 1164 Sofia, Bulgaria., Kukoc-Modun L; Department of Analytical Chemistry, Faculty of Chemistry and Technology, University of Split, Rudjera Boskovica 35, 21000 Split, Croatia., Kolev SD; Faculty of Chemistry and Pharmacy, Sofia University 'St. Kl. Ohridski', 1 James Bourchier Blvd., 1164 Sofia, Bulgaria.; School of Chemistry, The University of Melbourne, Parkville, VIC 3010, Australia.; Department of Chemical Engineering, The University of Melbourne, Parkville, VIC 3010, Australia.
Jazyk: angličtina
Zdroj: Sensors (Basel, Switzerland) [Sensors (Basel)] 2024 Aug 31; Vol. 24 (17). Date of Electronic Publication: 2024 Aug 31.
DOI: 10.3390/s24175677
Abstrakt: A sequential injection analysis method for the determination of glutathione (GSH) in pharmaceuticals has been developed. It is based on the reduction of the Cu(II)-neocuproine complex by GSH and the formation of an orange-yellow colored Cu(I)-neocuproine complex with maximum absorbance at 458 nm. Under optimal conditions the method is characterized by a linear calibration range of 6.0 × 10 -7 -8.0 × 10 -5 mol L -1 ( A max = 3270 C GSH - 0.0010; R 2 = 0.9983), limit of detection of 2.0 × 10 -7 mol L -1 , limit of quantification of 6.7 × 10 -7 mol L -1 , repeatability (expressed as relative standard deviation) of 3.8%, and sampling rate of 60 h -1 . The newly developed method has been successfully applied to the determination of GSH in pharmaceutical samples with no statistically significant difference between the results obtained and those produced by the standard Pharmacopoeia method.
Databáze: MEDLINE
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