| Autor: |
Libero ML; Department of Pharmacy, 'G. d'Annunzio' University, 66013 Chieti, Italy.; Department of Cell Biology, Physiology and Immunology, University of Cordoba, 14014 Cordoba, Spain.; Maimonides Biomedical Research Institute of Cordoba (IMIBIC), 14004 Cordoba, Spain., Montero-Hidalgo AJ; Department of Cell Biology, Physiology and Immunology, University of Cordoba, 14014 Cordoba, Spain.; Maimonides Biomedical Research Institute of Cordoba (IMIBIC), 14004 Cordoba, Spain.; Reina Sofia University Hospital (HURS), 14004 Cordoba, Spain.; Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y Nutrición (CIBERobn), 14004 Cordoba, Spain., Recinella L; Department of Pharmacy, 'G. d'Annunzio' University, 66013 Chieti, Italy., Luque RM; Department of Cell Biology, Physiology and Immunology, University of Cordoba, 14014 Cordoba, Spain.; Maimonides Biomedical Research Institute of Cordoba (IMIBIC), 14004 Cordoba, Spain.; Reina Sofia University Hospital (HURS), 14004 Cordoba, Spain.; Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y Nutrición (CIBERobn), 14004 Cordoba, Spain., Generali D; Department of Medical, Surgical and Health Sciences, University of Trieste, 34149 Trieste, Italy.; Department of Advanced Translational Microbiology, Institute for Maternal and Child Health-IRCCS 'Burlo Garofolo', 34137 Trieste, Italy., Acquaviva A; Department of Pharmacy, 'G. d'Annunzio' University, 66013 Chieti, Italy., Orlando G; Department of Pharmacy, 'G. d'Annunzio' University, 66013 Chieti, Italy., Ferrante C; Department of Pharmacy, 'G. d'Annunzio' University, 66013 Chieti, Italy., Menghini L; Department of Pharmacy, 'G. d'Annunzio' University, 66013 Chieti, Italy., Di Simone SC; Department of Pharmacy, 'G. d'Annunzio' University, 66013 Chieti, Italy., Nilofar N; Department of Pharmacy, 'G. d'Annunzio' University, 66013 Chieti, Italy., Chiavaroli A; Department of Pharmacy, 'G. d'Annunzio' University, 66013 Chieti, Italy., Brunetti L; Department of Pharmacy, 'G. d'Annunzio' University, 66013 Chieti, Italy., Leone S; Department of Pharmacy, 'G. d'Annunzio' University, 66013 Chieti, Italy. |
| Abstrakt: |
Chronic inflammation is a recognized risk factor for various cancers, including prostate cancer (PCa). We aim to explore the potential protective effects of aged black garlic extract (ABGE) against inflammation-induced prostate damage and its impact on prostate cancer cell lines. We used an ex vivo model of inflammation induced by Escherichia coli lipopolysaccharide (LPS) on C57BL/6 male mouse prostate specimens to investigate the anti-inflammatory properties of ABGE. The gene expression levels of pro-inflammatory biomarkers ( COX-2 , NF-κB , and TNF-α , IL-6 ) were measured. Additionally, we evaluated ABGE's therapeutic effects on the prostate cancer cell lines through in vitro functional assays, including colony formation, tumorsphere formation, migration assays, and phosphorylation arrays to assess the signaling pathways (MAPK, AKT, JAK/STAT, and TGF-β). ABGE demonstrated significant anti-inflammatory and antioxidant effects in preclinical models, partly attributed to its polyphenolic content, notably catechin and gallic acid. In the ex vivo model, ABGE reduced the gene expression levels of COX-2 , NF-κB , TNF-α , and IL-6 . The in vitro studies showed that ABGE inhibited cell proliferation, colony and tumorsphere formation, and cell migration in the prostate cancer cells, suggesting its potential as a therapeutic agent. ABGE exhibits promising anti-inflammatory and anti-cancer properties, supporting further investigation into ABGE as a potential agent for managing inflammation and prostate cancer. |
