| Autor: |
Zhao ZZ; School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China., Liang XB; School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China., He HJ; Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou 450046, China., Xue GM; School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China., Sun YJ; School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China., Chen H; School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China., Zhao YS; School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China., Bian LN; School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China., Feng WS; School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China., Zheng XK; School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China. |
| Abstrakt: |
Psoriasis, an immune-mediated inflammatory skin disorder, seriously affects the quality of life of nearly four percent of the world population. Euphorbia helioscopia L. is the monarch constituent of Chinese ZeQi powder preparation for psoriasis, so it is necessary to illustrate its active ingredients. Thus, twenty-three diterpenoids, including seven new ones, were isolated from the whole herb of E. helioscopia L. Compounds 1 and 2 , each featuring a 2,3-dicarboxylic functionality, are the first examples in the ent -2,3- sceo -atisane or the ent -2,3- sceo -abietane family. Extensive spectroscopic analysis (1D, 2D NMR, and HRMS data) and computational methods were used to confirm their structures and absolute configurations. According to the previous study and NMR data from the jatropha diterpenes obtained in this study, some efficient 1 H NMR spectroscopic rules for assigning the relative configurations of 3 α -benzyloxy-jatroph-11 E -ene and 7,8- seco -3 α -benzyloxy-jatropha-11 E -ene were summarized. Moreover, the hyperproliferation of T cells and keratinocytes is considered a key pathophysiology of psoriasis. Anti-proliferative activities against induced T/B lymphocytes and HaCaT cells were tested, and IC 50 values of some compounds ranged from 6.7 to 31.5 μM. Compounds 7 and 11 reduced the secretions of IFN- γ and IL-2 significantly. Further immunofluorescence experiments and a docking study with NF- κ B P65 showed that compound 13 interfered with the proliferation of HaCaT cells by inhibiting the NF- κ B P65 phosphorylation at the protein level. |
