| Autor: |
Friščić M; Department of Pharmaceutical Botany, University of Zagreb Faculty of Pharmacy and Biochemistry, Ante Kovačića 1, 10 000 Zagreb, Croatia., Maleš Ž; Department of Pharmaceutical Botany, University of Zagreb Faculty of Pharmacy and Biochemistry, Ante Kovačića 1, 10 000 Zagreb, Croatia., Maleš I; Department of Pharmacy, University of Split School of Medicine, Šoltanska 2A, 21 000 Split, Croatia., Duka I; Department of Pharmaceutical Botany, University of Zagreb Faculty of Pharmacy and Biochemistry, Ante Kovačića 1, 10 000 Zagreb, Croatia.; Medical School Karlovac, Dr Andrije Štampara 5, 47 000 Karlovac, Croatia., Radonić A; Department of Organic Chemistry, University of Split Faculty of Chemistry and Technology, Ruđera Boškovića 35, 21 000 Split, Croatia., Mitić B; Division of Botany, Department of Biology, University of Zagreb Faculty of Science, Marulićev trg 9a, 10 000 Zagreb, Croatia., Hruševar D; Division of Botany, Department of Biology, University of Zagreb Faculty of Science, Marulićev trg 9a, 10 000 Zagreb, Croatia., Jurić S; Department of Organic Chemistry, University of Zagreb Faculty of Pharmacy and Biochemistry, Ante Kovačića 1, 10 000 Zagreb, Croatia., Jerković I; Department of Organic Chemistry, University of Split Faculty of Chemistry and Technology, Ruđera Boškovića 35, 21 000 Split, Croatia. |
| Abstrakt: |
Iris taxa are sources of valuable essential oils obtained from aged rhizomes used by various industries, including pharmacy, cosmetic, perfume, and food industry, in which irones are the most important aroma components. In this study, volatile organic compounds (VOCs) obtained from dried rhizomes of three endemics from Croatia, Iris pseudopallida , I. illyrica , and I. adriatica , were studied. The VOCs were isolated by three different methods: headspace solid-phase microextraction (HS-SPME) using divinylbenzene/carboxene/polydimethylsiloxane (DVB/CAR/PDMS) fiber or polydimethylsiloxane/divinylbenzene (PDMS/DVB) fiber, and hydrodistillation (HD). The samples were analyzed by gas chromatography-mass spectrometry (GC-MS). In five out of six samples, the main compounds detected by HS-SPME were perilla aldehyde, butan-2,3-diol, acetic acid, 2-phenylethanol, benzyl alcohol, hexanal, and nonanal, while 6-methylhept-5-en-2-one, trans -caryophyllene, and ethanol were common for all studied samples. The former VOCs were absent from the oldest, irone-rich I. pseudopallida sample, mainly characterized by cis -α-irone (43.74-45.76%). When using HD, its content was reduced (24.70%), while docosane prevailed (45.79%). HD yielded predominantly fatty acids, including myristic, common for all studied taxa (4.20-97.01%), and linoleic (40.69%) and palmitic (35.48%) as the major VOCs of I. adriatica EO. The performed GC-MS analyses of EOs, in combination with HS-SPME/GC-MS, proved to be useful for gaining a better insight into Iris VOCs. |
