| Autor: |
Lin CH; Department of Forestry, National Chung Hsing University, Taichung 402202, Taiwan.; Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung 404328, Taiwan., Chiang YT; Department of Forestry, National Chung Hsing University, Taichung 402202, Taiwan.; Liyu International Co., Ltd., Taichung 402202, Taiwan., Lin LY; Liyu International Co., Ltd., Taichung 402202, Taiwan., Tsao NW; Special Crop and Metabolome Discipline Cluster, Academy Circle Economy, National Chung Hsing University, Taichung 402202, Taiwan., Wang CH; Special Crop and Metabolome Discipline Cluster, Academy Circle Economy, National Chung Hsing University, Taichung 402202, Taiwan., Chien SC; Experimental Forest Management Office, National Chung-Hsing University, Taichung 402202, Taiwan., Sun YH; Department of Forestry, National Chung Hsing University, Taichung 402202, Taiwan., Wang SY; Department of Forestry, National Chung Hsing University, Taichung 402202, Taiwan.; Special Crop and Metabolome Discipline Cluster, Academy Circle Economy, National Chung Hsing University, Taichung 402202, Taiwan.; Agricultural Biotechnology Research Center, Academia Sinica, Taipei City 115201, Taiwan. |
| Abstrakt: |
This study investigates the composition characteristics and anti-inflammatory activity mechanisms of the essential oil from the leaves of Crossostephium chinense . C. chinense is a perennial herb commonly found in East Asia, traditionally used to treat various ailments. The essential oil extracted through water distillation, primarily contains 1,8-cineole (13.73%), santolina triene (13.53%), and germacrene D (10.67%). Three compounds were identified from the essential oil, namely 1-acetoxy-2-(2-hydroxypropyl)-5-methylhex-3,5-diene, 1-acetoxy-isopyliden-hex-5-en-4-one, and chrysanthemyl acetate, with the first two being newly discovered compounds. Then, the essential oil of C. chinense exhibits significant anti-inflammatory effects on RAW264.7 macrophages, effectively inhibiting the production of NO and ROS, with the IC50 value of 10.3 μg/mL. Furthermore, the essential oil reduces the expression of pro-inflammatory cytokines such as TNF-α, IL-6, and IL-1β. Mechanistic studies indicate that the essential oil affects the inflammatory response by inhibiting the expression of iNOS but has no significant impact on COX-2. Further analysis suggests that the essential oil may regulate the inflammatory response through the ERK protein in the MAPK pathway and IκBα in the NF-κB pathway, while also promoting the activity of the NRF2/HO-1 antioxidant pathway, enhancing the cell's antioxidant capacity, thereby achieving an effect of inhibiting the inflammatory response. These results highlight the potential application value of C. chinense leaf essential oil in the medical and healthcare fields. |
