Autor: |
Pishchik VN; All-Russia Research Institute for Agricultural Microbiology, Podbelskogo hwy 3, Pushkin, 196608 St. Petersburg, Russia., Chizhevskaya EP; All-Russia Research Institute for Agricultural Microbiology, Podbelskogo hwy 3, Pushkin, 196608 St. Petersburg, Russia., Kichko AA; All-Russia Research Institute for Agricultural Microbiology, Podbelskogo hwy 3, Pushkin, 196608 St. Petersburg, Russia., Aksenova TS; All-Russia Research Institute for Agricultural Microbiology, Podbelskogo hwy 3, Pushkin, 196608 St. Petersburg, Russia., Andronov EE; All-Russia Research Institute for Agricultural Microbiology, Podbelskogo hwy 3, Pushkin, 196608 St. Petersburg, Russia., Chebotar VK; All-Russia Research Institute for Agricultural Microbiology, Podbelskogo hwy 3, Pushkin, 196608 St. Petersburg, Russia., Filippova PS; St. Petersburg North-West Centre of Interdisciplinary Researches of Problems of Food Maintenance, Podbelskogo hwy, 7, Pushkin, 196608 St. Petersburg, Russia., Shelenga TV; Federal Center N. I. Vavilov All-Russian Institute of Plant Genetic Resources, Bolshaya Morskaya Street, 44, 190121 St. Petersburg, Russia., Belousova MH; Federal Center N. I. Vavilov All-Russian Institute of Plant Genetic Resources, Bolshaya Morskaya Street, 44, 190121 St. Petersburg, Russia., Chikida NN; Federal Center N. I. Vavilov All-Russian Institute of Plant Genetic Resources, Bolshaya Morskaya Street, 44, 190121 St. Petersburg, Russia. |
Abstrakt: |
The present study demonstrated the differences in the seed metabolome and mycobiome of two Aegilops tauschii Coss accessions with different resistance to brown rust and powdery mildew. We hypothesized that the seeds of resistant accession k-1958 Ae. tauschii ssp. strangulata can contain a larger number of metabolites with antifungal activity compared with the seeds of susceptible Ae. tauschii ssp meyeri k-340, which will determine differences in the seed fungal community. Our study emphasizes the differences in the seed metabolome of the studied Ae. tauschii accessions. The resistant accession k-1958 had a higher content of glucose and organic acids, including pyruvic, salicylic and azelaic acid, as well as pipecolic acids, galactinol, glycerol and sitosterol. The seeds of Ae. tauschii -resistant accession k-1958 were found to contain more active substances with antifungal activity. The genera Cladosporium and Alternaria were dominant in the seed mycobiome of the resistant accession. The genera Alternaria , Blumeria and Cladosporium dominated in seed mycobiome of susceptible accession k-340. In the seed mycobiome of the resistant k-1958, a higher occurrence of saprotrophic micromycetes was found, and many of the micromycetes were biocontrol agents. It was concluded that differences in the seed metabolome of Ae. tauschii contributed to the determination of the differences in mycobiomes. |