Genomics, Proteomics, and Antifungal Activity of Chitinase from the Antarctic Marine Bacterium Curtobacterium sp. CBMAI 2942.

Autor: Vasquez YMS; Divisão de Recursos Microbianos, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA), Universidade Estadual de Campinas (UNICAMP), Paulínia 13148-218, SP, Brazil.; Institute of Biology, Campinas State University (UNICAMP), Campinas 13083-970, SP, Brazil., Cueva-Yesquen LG; Divisão de Recursos Microbianos, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA), Universidade Estadual de Campinas (UNICAMP), Paulínia 13148-218, SP, Brazil.; Institute of Biology, Campinas State University (UNICAMP), Campinas 13083-970, SP, Brazil., Duarte AWF; Complexo de Ciências Médicas e de Enfermagem, Universidade Federal de Alagoas, Campus Arapiraca, Arapiraca 57309-005, AL, Brazil., Rosa LH; Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, Brazil., Valladão R; Laboratory of Biochemistry, Instituto Butantan, São Paulo 05585-000, SP, Brazil., Lopes AR; Laboratory of Biochemistry, Instituto Butantan, São Paulo 05585-000, SP, Brazil., Costa Bonugli-Santos R; Instituto Latino Americano de Ciências da Vida e da Natureza (ILACVN), Universidade Federal da Integração Latino-Americana (UNILA), Foz do Iguaçu 85870-650, PR, Brazil., Oliveira VM; Divisão de Recursos Microbianos, Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e Agrícolas (CPQBA), Universidade Estadual de Campinas (UNICAMP), Paulínia 13148-218, SP, Brazil.
Jazyk: angličtina
Zdroj: International journal of molecular sciences [Int J Mol Sci] 2024 Aug 26; Vol. 25 (17). Date of Electronic Publication: 2024 Aug 26.
DOI: 10.3390/ijms25179250
Abstrakt: This study aimed to evaluate the genomic profile of the Antarctic marine Curtobacterium sp. CBMAI 2942, as well as to optimize the conditions for chitinase production and antifungal potential for biological control. Assembly and annotation of the genome confirmed the genomic potential for chitinase synthesis, revealing two ChBDs of chitin binding (Chi C). The optimization enzyme production using an experimental design resulted in a 3.7-fold increase in chitinase production. The chitinase enzyme was identified by SDS-PAGE and confirmed through mass spectrometry analysis. The enzymatic extract obtained using acetone showed antifungal activity against the phytopathogenic fungus Aspergillus sp. series Nigri CBMAI 1846. The genetic capability of Curtobacterium sp. CBMAI 2942 for chitin degradation was confirmed through genomic analysis. The basal culture medium was adjusted, and the chitinase produced by this isolate from Antarctica showed significant inhibition against Aspergillus sp. Nigri series CBMAI 1846, which is a tomato phytopathogenic fungus. This suggests that this marine bacterium could potentially be used as a biological control of agricultural pests.
Databáze: MEDLINE
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