| Autor: |
Abdoudaim MS; Unité d'Epidémiologie Moléculaire et Diversité des Microorganismes, Faculté des Sciences et Techniques, Université de Nouakchott, Nouakchott 2373, Mauritania.; Laboratoire de Virologie, Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, 75015 Paris, France., Mohamed Abdellahi MV; Unité d'Epidémiologie Moléculaire et Diversité des Microorganismes, Faculté des Sciences et Techniques, Université de Nouakchott, Nouakchott 2373, Mauritania., Mohamed Baba ND; Centre Hospitalier National, Nouakchott 5026, Mauritania., Mboumba Bouassa RS; Laboratoire de Virologie, Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, 75015 Paris, France.; Department of Family Medicine, Faculty of Medicine, Institut du Savoir Montfort, Montfort Hospital, University of Ottawa, Ottawa, ON K1K 0T2, Canada.; Ecole Doctorale Régionale (EDR) d'Afrique Centrale en Infectiologie Tropicale, Franceville BP 876, Gabon., Ahmed MLCB; Unité d'Epidémiologie Moléculaire et Diversité des Microorganismes, Faculté des Sciences et Techniques, Université de Nouakchott, Nouakchott 2373, Mauritania., Bélec L; Laboratoire de Virologie, Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, 75015 Paris, France.; Faculté de Médecine Paris Descartes, Université Paris Cité, 75006 Paris, France. |
| Abstrakt: |
Cervical cancer related to high-risk human papillomavirus (HR-HPV) is the second female cancer in Mauritania (Northwest Sahelian Africa). We assessed the distribution of HPV genotypes in Mauritanian women with high-grade cervical intraepithelial neoplasia (CIN2/3) or invasive cervical cancer (ICC). A prospective study was conducted in the Centre Hospitalier National, Nouakchott, Mauritania, to collect cervical biopsies among women suspected of CIN2/3 or cancer. HPV DNA detection and genotyping were carried out from formalin-fixed, paraffin-embedded biopsies using multiplex PCR (Human Papillomavirus Genotyping Real-Time PCR Kit, Bioperfectus Technologies Co., Taizhou, China). Fifty biopsies were included from women (mean age: 56.7 years) suffering from CIN2/3 (28.0%) and ICC (72.0%) which corresponded to 32 (64.0%) squamous cell carcinomas (SCC) and 4 (8.0%) adenocarcinomas (ADC). HPV DNA detection was successful in 47 (94.0%) samples. The most prevalent HR-HPV genotypes were HPV-45 (40.4%), HPV-16 (38.3%), HPV-39 and HPV-52 (23.4%), HPV-33 (17.0%), HPV-18 (14.9%), HPV-35 (4.2%), and HPV-56 (2.1%). The majority (93.6%) of HPV-positive biopsies contained at least one HPV type covered by the 9-valent Gardasil-9 ® vaccine, and 40.9% were infected by multiple vaccine HPV genotypes. To eradicate cervical cancer in Mauritania, prophylactic HPV vaccination must be combined with primary molecular screening of cervical HR-HPV infection. |
