MASTER-NAADP: a membrane permeable precursor of the Ca 2+ mobilizing second messenger NAADP.

Autor: Krukenberg S; Organic Chemistry, University of Hamburg, 20146, Hamburg, Germany., Möckl F; The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Weiß M; The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Dekiert P; Organic Chemistry, University of Hamburg, 20146, Hamburg, Germany., Hofmann M; Organic Chemistry, University of Hamburg, 20146, Hamburg, Germany., Gerlach F; The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Winterberg KJ; The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Kovacevic D; The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Khansahib I; The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Troost B; The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Hinrichs M; The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Granato V; The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Nawrocki M; Section of Molecular Immunology and Gastroenterology, I. Department of Medicine, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Hub T; Institute of Pharmacology, Heidelberg University, Heidelberg, Germany.; DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, Heidelberg, Germany., Tsvilovskyy V; Institute of Pharmacology, Heidelberg University, Heidelberg, Germany.; DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, Heidelberg, Germany., Medert R; Institute of Pharmacology, Heidelberg University, Heidelberg, Germany.; DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, Heidelberg, Germany., Woelk LM; Department of Applied Medical Informatics, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Förster F; Department of Applied Medical Informatics, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Li H; Department of Anesthesiology, Columbia University Irving Medical Center, New York, NY, USA.; Department of Physiology and Cellular Biophysics, Columbia University, New York, NY, USA., Werner R; Department of Applied Medical Informatics, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Altfeld M; Department of Immunology, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Huber S; Section of Molecular Immunology and Gastroenterology, I. Department of Medicine, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Clarke OB; Department of Anesthesiology, Columbia University Irving Medical Center, New York, NY, USA.; Department of Physiology and Cellular Biophysics, Columbia University, New York, NY, USA., Freichel M; Institute of Pharmacology, Heidelberg University, Heidelberg, Germany.; DZHK (German Centre for Cardiovascular Research), partner site Heidelberg/Mannheim, Heidelberg, Germany., Diercks BP; The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany., Meier C; Organic Chemistry, University of Hamburg, 20146, Hamburg, Germany., Guse AH; The Calcium Signalling Group, Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, 20246, Hamburg, Germany. guse@uke.de.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Sep 13; Vol. 15 (1), pp. 8008. Date of Electronic Publication: 2024 Sep 13.
DOI: 10.1038/s41467-024-52024-y
Abstrakt: Upon stimulation of membrane receptors, nicotinic acid adenine dinucleotide phosphate (NAADP) is formed as second messenger within seconds and evokes Ca 2+ signaling in many different cell types. Here, to directly stimulate NAADP signaling, MASTER-NAADP, a Membrane permeAble, STabilized, bio-rEversibly pRotected precursor of NAADP is synthesized and release of its active NAADP mimetic, benzoic acid C-nucleoside, 2'-phospho-3'F-adenosine-diphosphate, by esterase digestion is confirmed. In the presence of NAADP receptor HN1L/JPT2 (hematological and neurological expressed 1-like protein, HN1L, also known as Jupiter microtubule-associated homolog 2, JPT2), this active NAADP mimetic releases Ca 2+ and increases the open probability of type 1 ryanodine receptor. When added to intact cells, MASTER-NAADP initially evokes single local Ca 2+ signals of low amplitude. Subsequently, also global Ca 2+ signaling is observed in T cells, natural killer cells, and Neuro2A cells. In contrast, control compound MASTER-NADP does not stimulate Ca 2+ signaling. Likewise, in cells devoid of HN1L/JPT2, MASTER-NAADP does not affect Ca 2+ signaling, confirming that the product released from MASTER-NAADP is a bona fide NAADP mimetic.
(© 2024. The Author(s).)
Databáze: MEDLINE
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