Diagnostic potential of combining plasma biomarkers of tissue damage and inflammation in pediatric TB.
Autor: | López-Suárez A; Pediatric Infectious Diseases Department, Gregorio Marañón University Hospital, Madrid, Spain; Biomedical Research Centre Network for Infectious Diseases (CIBERINFEC), Carlos III Health Institute, Madrid, Spain. Electronic address: aalopezsuarez@gmail.com., Santos-Sebastián M; Pediatric Infectious Diseases Department, Gregorio Marañón University Hospital, Madrid, Spain; Biomedical Research Centre Network for Infectious Diseases (CIBERINFEC), Carlos III Health Institute, Madrid, Spain; Gregorio Marañón Research Health Institute (IiSGM), Madrid, Spain., Hernanz-Lobo A; Pediatric Infectious Diseases Department, Gregorio Marañón University Hospital, Madrid, Spain; Biomedical Research Centre Network for Infectious Diseases (CIBERINFEC), Carlos III Health Institute, Madrid, Spain., Rincón-López E; Pediatric Infectious Diseases Department, Gregorio Marañón University Hospital, Madrid, Spain; Biomedical Research Centre Network for Infectious Diseases (CIBERINFEC), Carlos III Health Institute, Madrid, Spain; Gregorio Marañón Research Health Institute (IiSGM), Madrid, Spain., Aguilera-Alonso D; Pediatric Infectious Diseases Department, Gregorio Marañón University Hospital, Madrid, Spain; Biomedical Research Centre Network for Infectious Diseases (CIBERINFEC), Carlos III Health Institute, Madrid, Spain; Gregorio Marañón Research Health Institute (IiSGM), Madrid, Spain., Saavedra-Lozano J; Pediatric Infectious Diseases Department, Gregorio Marañón University Hospital, Madrid, Spain; Biomedical Research Centre Network for Infectious Diseases (CIBERINFEC), Carlos III Health Institute, Madrid, Spain; Gregorio Marañón Research Health Institute (IiSGM), Madrid, Spain., Ruiz Serrano MJ; Microbiology and Infectious Diseases Department, Gregorio Marañón University Hospital, Madrid, Spain; Biomedical Research Centre Network for Respiratory Diseases (CIBERES), Carlos III Health Institute, Madrid, Spain., Hernández-Bartolomé Á; La Princesa Health Research Institute, IIS-IP, Madrid, Spain., Medrano de Dios LM; Unidad de Infección Viral e Inmunidad, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Madrid, Spain., Jiménez Fuentes JL; Gregorio Marañón Research Health Institute (IiSGM), Madrid, Spain; Laboratory Platform (Immunology), General Universitary Hospital Gregorio Marañon (HGUGM), Madrid, 28007, Spain; Spanish HIV HGM BioBank, Madrid, 28007, Spain., Navarro ML; Pediatric Infectious Diseases Department, Gregorio Marañón University Hospital, Madrid, Spain; Biomedical Research Centre Network for Infectious Diseases (CIBERINFEC), Carlos III Health Institute, Madrid, Spain; Gregorio Marañón Research Health Institute (IiSGM), Madrid, Spain., Tebruegge M; Department of Infection, Immunity & Inflammation, UCL Great Ormond Street Institute of Child Health, University College London, London, United Kingdom; Department of Paediatrics and National Reference Centre for Paediatric Tuberculosis, Klinik Ottakring, Wiener Gesundheitsverbund, Vienna, Austria; Department of Paediatrics, Royal Children's Hospital Melbourne, University of Melbourne, Melbourne, Australia., Santiago-García B; Pediatric Infectious Diseases Department, Gregorio Marañón University Hospital, Madrid, Spain; Biomedical Research Centre Network for Infectious Diseases (CIBERINFEC), Carlos III Health Institute, Madrid, Spain; Gregorio Marañón Research Health Institute (IiSGM), Madrid, Spain. |
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Jazyk: | angličtina |
Zdroj: | Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi [J Microbiol Immunol Infect] 2024 Dec; Vol. 57 (6), pp. 937-946. Date of Electronic Publication: 2024 Sep 06. |
DOI: | 10.1016/j.jmii.2024.07.011 |
Abstrakt: | Introduction: Immune-based diagnostic tests for tuberculosis (TB) have suboptimal sensitivity in children and cannot differentiate between latent infection (LTBI) and active disease. This study evaluated the diagnostic potential of a broad range of biomarkers of tissue damage and inflammation in unstimulated plasma in children. Methods: We analyzed 17 biomarkers in 15 non-M. tuberculosis (MTB)-infected controls and 33 children with TB infection (LTBI, n = 8; probable TB, n = 19; confirmed TB, n = 6). Biomarker concentrations were measured using a Luminex magnetic bead-based platform and multiplex sandwich immunoassays. Concentrations, correlations and diagnostic accuracy assessments were conducted among patient groups. Results: Confirmed TB cases had significantly higher concentrations of IFN-γ and IL-2 and higher IFN-γ/MCP-1 and IL-2/MCP-1 ratios compared to LTBI and non-MTB-infected children. Among children with confirmed TB, there was a strong correlation between IFN-γ and IL-10 (r = 0.95; p < 0.001) and a significant correlation between IL-2 and IL-1ra (r = 0.92), IL-21 (r = 0.91), MCP-3 (r = 0.84), and MMP-1 (r = 0.85). The IFN-γ/MCP-1 ratio was the most accurate biomarker combination for differentiating between MTB-infected and non-MTB-infected children (AUC, 0.82; sensitivity, 87.9%; specificity, 66.6%; p < 0.001) and between active TB and non-MTB-infected children (AUC 0.82; sensitivity 88.0%; specificity 60.0%; p < 0.001). None of the biomarkers investigated were able to discriminate between LTBI and active TB. Conclusion: Our data suggest that combining the analyses of multiple biomarkers in plasma has the potential to enhance diagnosis of TB in children and, thus, warrants additional investigation. In particular, the diagnostic potential of IFN-γ/MCP-1 ratios should be further explored in larger pediatric cohorts. (Copyright © 2024. Published by Elsevier B.V.) |
Databáze: | MEDLINE |
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