Effects of perfluoroalkyl sulfonic acids on developmental, physiological, and immunological measures in northern leopard frog tadpoles.

Autor: Rohonczy J; Department of Biology, Carleton University, Ottawa, ON, K1S 5B6, Canada., Forbes MR; Department of Biology, Carleton University, Ottawa, ON, K1S 5B6, Canada., Gilroy ÈAM; Water Science and Technology Directorate, Environment and Climate Change Canada, Burlington, ON, L7S 1A1, Canada., Carpenter DJ; Wildlife and Landscape Science Directorate, Environment and Climate Change Canada, Ottawa, ON, K1A 0H3, Canada., Young SD; Wildlife and Landscape Science Directorate, Environment and Climate Change Canada, Ottawa, ON, K1A 0H3, Canada., Morrill A; Wildlife and Landscape Science Directorate, Environment and Climate Change Canada, Ottawa, ON, K1A 0H3, Canada., Brinovcar C; Water Science and Technology Directorate, Environment and Climate Change Canada, Burlington, ON, L7S 1A1, Canada., De Silva AO; Water Science and Technology Directorate, Environment and Climate Change Canada, Burlington, ON, L7S 1A1, Canada., Bartlett AJ; Water Science and Technology Directorate, Environment and Climate Change Canada, Burlington, ON, L7S 1A1, Canada., Robinson SA; Department of Biology, Carleton University, Ottawa, ON, K1S 5B6, Canada; Wildlife and Landscape Science Directorate, Environment and Climate Change Canada, Ottawa, ON, K1A 0H3, Canada. Electronic address: Stacey.Robinson@ec.gc.ca.
Jazyk: angličtina
Zdroj: Chemosphere [Chemosphere] 2024 Oct; Vol. 365, pp. 143333. Date of Electronic Publication: 2024 Sep 11.
DOI: 10.1016/j.chemosphere.2024.143333
Abstrakt: The chronic toxicity of short chain perfluoroalkyl sulfonic acids (PFSAs), such as perfluorobutanesulfonic acid (PFBS) and perfluorohexanesulfonic acid (PFHxS), are relatively understudied despite the increasing detection of these compounds in the environment. We investigated the chronic toxicity and bioconcentration of PFBS and PFHxS using northern leopard frog (Rana [Lithobates] pipiens) tadpoles. We exposed Gosner stage (GS) 25 tadpoles to either PFBS or PFHxS at nominal concentrations of 0.1, 1, 10, 100, and 1000 μg/L until metamorphosis (GS42). We then assessed tadpole growth, development, stress, and immune metrics, and measured fatty acid (FA) composition and PFSA concentrations in liver and whole-body tissues. Tadpole growth and development measures were relatively unaffected by PFSA exposure. However, tadpoles exposed to 1000 μg/L PFBS or PFHxS had significantly increased hepatosomatic indexes (HSI) relative to controls. Further, tadpoles from the 1000 μg/L PFHxS treatment had altered FA profiles relative to controls, with increased total FAs, saturated FAs, monounsaturated FAs, and omega-6 polyunsaturated FAs. In addition, tadpoles from the 1000 μg/L PFHxS treatment had a higher probability of waterborne corticosterone detection. These results suggest that PFBS and PFHxS influence the hepatic health of tadpoles, and that PFHxS may alter lipid metabolism in tadpoles. We also observed a higher probability of tadpoles being phenotypically female after exposure to an environmentally relevant concentration (0.1 μg/L) of PFHxS, suggesting that PFHxS may exert endocrine disrupting effects on tadpoles during early development. The measured bioconcentration factors (BCFs) for both compounds were ≤10 L kg -1 wet weight, suggesting low bioconcentration potential for PFBS and PFHxS in tadpoles. Many of the significant effects observed in this study occurred at concentrations several orders of magnitude above those measured in the environment; however, our work shows effects of PFSAs exposure on amphibians and provides essential information for ecological risk assessments of these compounds.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Crown Copyright © 2024. Published by Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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