Sulfated polysaccharide from Antrodia cinnamomea mycelium cultured with zinc sulfate stimulates M1 polarization of macrophages through AKT/mTOR pathways.

Autor: Lin ZH; Institute of Traditional Medicine, National Yang Ming Chiao Tung University, 155 Li-Nung St., Sec. 2, Shipai, Beitou, Taipei 112, Taiwan., Lo HC; Institute of Traditional Medicine, National Yang Ming Chiao Tung University, 155 Li-Nung St., Sec. 2, Shipai, Beitou, Taipei 112, Taiwan; Program in Molecular Medicine, National Yang Ming Chiao Tung University and Academia Sinica, Taipei, Taiwan., Chang CC; School of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan., Lu MK; Institute of Traditional Medicine, National Yang Ming Chiao Tung University, 155 Li-Nung St., Sec. 2, Shipai, Beitou, Taipei 112, Taiwan; School of Chinese Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; National Research Institute of Chinese Medicine, Ministry of Health and Welfare, 155-1 Li-Nung St., Sec. 2, Shipai, Beitou, Taipei 112, Taiwan; Traditional Chinese Medicine Glycomics Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan. Electronic address: meiklu@nycu.edu.tw., Tseng AJ; Institute of Traditional Medicine, National Yang Ming Chiao Tung University, 155 Li-Nung St., Sec. 2, Shipai, Beitou, Taipei 112, Taiwan., Chao CH; School of Chinese Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan., Chao CH; Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu, Taiwan; Center for Intelligent Drug Systems and Smart Bio-devices (IDS2B), National Yang Ming Chiao Tung University, Hsinchu, Taiwan., Lin TY; Institute of Traditional Medicine, National Yang Ming Chiao Tung University, 155 Li-Nung St., Sec. 2, Shipai, Beitou, Taipei 112, Taiwan; Program in Molecular Medicine, National Yang Ming Chiao Tung University and Academia Sinica, Taipei, Taiwan; School of Chinese Medicine, National Yang Ming Chiao Tung University, Taipei, Taiwan; Traditional Chinese Medicine Glycomics Research Center, National Yang Ming Chiao Tung University, Taipei, Taiwan. Electronic address: tylin99@nycu.edu.tw.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Nov; Vol. 279 (Pt 4), pp. 135548. Date of Electronic Publication: 2024 Sep 11.
DOI: 10.1016/j.ijbiomac.2024.135548
Abstrakt: Antrodia cinnamomea-derived sulfated polysaccharides (Ac-SPSs) have health benefits, but their yield is low. This study explores a strategy to increase Ac-SPS yield and elucidates the biofunctions of Ac-SPS. For this, A. cinnamomea mycelia were treated with zinc sulfate (ZnSO 4 ) administered at 1, 10, and 100 μM. Firstly, functional assay indicated that ZnSO 4 increases the Ac-SPS yield by 20 %-30 % compared with the control treatment. ZnSO 4 engenders a population of middle-molecular-weight (~200 kDa) Ac-SPSs. Ac-SPS (ASZ-10) from A. cinnamomea treated with 10 μM ZnSO 4 exhibits the best anti-proliferation ability against lung cancer A549 cells. Co-treatment of ASZ-10 does not inhibit lipopolysaccharide-induced inflammation but does induce M1-related markers of macrophage RAW264.7 cells. Secondly, immunomodulatory properties showed that ASZ-10 increases the expression of CD80 + and CD86 + in M-CSF-stimulated bone-marrow-derived macrophages. ASZ-10 induces M1 polarization through up-regulation of the AKT/mTOR pathway as confirmed by AKT and mTOR inhibitors eliminating ASZ-10-induced M1-like markers of macrophages. Through systemic chemical and functional analysis, this study shows that trace amounts (10 μM) of ZnSO 4 increase Ac-SPS yield and it reveals that ASZ-10 exhibits anti-cancer activity and acts as a stimulator for M1 macrophages by stimulation of AKT and mTOR.
Competing Interests: Declaration of competing interest The authors declare no conflicts of interest. Content will be included as a part of Zhi-Hu Lin's doctoral thesis at the Institute of Traditional Medicine, College of Medicine, National Yang Ming Chiao Tung University.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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