New commercial wipes inhibit the dispersion and adhesion of Staphylococcus aureus and Pseudomonas aeruginosa biofilms.

Autor:	Di Fermo P; Department of Medical, Oral and Biotechnological Sciences, University of 'G. d'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Diban F; Department of Pharmacy, University of 'G. d'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Ancarani E; Lombarda H. S.r.l., Albairate, 20080 Milan, Italy., Yu K; Shanghai Joy Crown Industry Co., Ltd, 200125 Shanghai, China., D'Arcangelo S; Department of Pharmacy, University of 'G. d'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., D'Ercole S; Department of Medical, Oral and Biotechnological Sciences, University of 'G. d'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Di Lodovico S; Department of Pharmacy, University of 'G. d'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Di Giulio M; Department of Pharmacy, University of 'G. d'Annunzio' Chieti-Pescara, 66100 Chieti, Italy., Cellini L; Department of Pharmacy, University of 'G. d'Annunzio' Chieti-Pescara, 66100 Chieti, Italy.
Jazyk:	angličtina
Zdroj:	Journal of applied microbiology [J Appl Microbiol] 2024 Sep 02; Vol. 135 (9).
DOI:	10.1093/jambio/lxae234
Abstrakt:	Aim: Bacterial biofilms can form on surfaces in hospitals, clinics, farms, and food processing plants, representing a possible source of infections and cross-contamination. This study investigates the effectiveness of new commercial wipes against Staphylococcus aureus and Pseudomonas aeruginosa biofilms (early attachment and formed biofilms), assessing LH SALVIETTE wipes (Lombarda H S.r.l.) potential for controlling biofilm formation. Methods and Results: The wipes efficacy was studied against the early attachment phase and formed biofilm of S. aureus ATCC 6538 and P. aeruginosa ATCC 15442 on a polyvinyl chloride (PVC) surface, following a modified standard test EN 16615:2015, measuring Log10 reduction and cell viability using live/dead staining. It was also evaluated the wipes anti-adhesive activity over time (3 h, 2 4h), calculating CFU.mL-1 reduction. Data were analyzed using t-student test. The wipes significantly reduced both early phase and formed S. aureus biofilm, preventing dispersion on PVC surfaces. Live/dead imaging showed bacterial cluster disaggregation and killing action. The bacterial adhesive capability decreased after short-time treatment (3 h) with the wipes compared to 24 h. Conclusions: Results demonstrated decreased bacterial count on PVC surface both for early attachment phase and formed biofilms, also preventing the bacterial biofilm dispersion. (© The Author(s) 2024. Published by Oxford University Press on behalf of Applied Microbiology International.)
Databáze:	MEDLINE
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