Microbial green synthesis of luminescent terbium sulfide nanoparticles using E. Coli: a rare earth element detoxification mechanism.

Autor: León JJ; BioNanotechnology and Microbiology Laboratory, Center for Bioinformatics and Integrative Biology (CBIB), Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Chile.; Department of Chemistry and the Institute for Lasers, Photonics, and Biophotonics, University at Buffalo, State University of New York, Buffalo, NY, USA.; Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY, USA., Oetiker N; BioNanotechnology and Microbiology Laboratory, Center for Bioinformatics and Integrative Biology (CBIB), Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Chile.; Department of Chemistry and the Institute for Lasers, Photonics, and Biophotonics, University at Buffalo, State University of New York, Buffalo, NY, USA.; Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY, USA., Torres N; BioNanotechnology and Microbiology Laboratory, Center for Bioinformatics and Integrative Biology (CBIB), Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Chile., Bruna N; BioNanotechnology and Microbiology Laboratory, Center for Bioinformatics and Integrative Biology (CBIB), Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Chile., Oskolkov E; BioNanotechnology and Microbiology Laboratory, Center for Bioinformatics and Integrative Biology (CBIB), Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Chile., Lei P; Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY, USA., Kuzmin AN; Department of Chemistry and the Institute for Lasers, Photonics, and Biophotonics, University at Buffalo, State University of New York, Buffalo, NY, USA., Chen K; Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY, USA., Andreadis S; Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY, USA., Pfeifer BA; Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY, USA., Swihart MT; Department of Chemical and Biological Engineering, University at Buffalo, State University of New York, Buffalo, NY, USA., Prasad PN; Department of Chemistry and the Institute for Lasers, Photonics, and Biophotonics, University at Buffalo, State University of New York, Buffalo, NY, USA. pnprasad@buffalo.edu., Pérez-Donoso J; BioNanotechnology and Microbiology Laboratory, Center for Bioinformatics and Integrative Biology (CBIB), Facultad de Ciencias de la Vida, Universidad Andres Bello, Santiago, Chile. jperezd@gmail.com.
Jazyk: angličtina
Zdroj: Microbial cell factories [Microb Cell Fact] 2024 Sep 12; Vol. 23 (1), pp. 248. Date of Electronic Publication: 2024 Sep 12.
DOI: 10.1186/s12934-024-02519-6
Abstrakt: Background: Rare-earth sulfide nanoparticles (NPs) could harness the optical and magnetic features of rare-earth ions for applications in nanotechnology. However, reports of their synthesis are scarce and typically require high temperatures and long synthesis times.
Results: Here we present a biosynthesis of terbium sulfide (TbS) NPs using microorganisms, identifying conditions that allow Escherichia coli to extracellularly produce TbS NPs in aqueous media at 37 °C by controlling cellular sulfur metabolism to produce a high concentration of sulfide ions. Electron microscopy revealed ultrasmall spherical NPs with a mean diameter of 4.1 ± 1.3 nm. Electron diffraction indicated a high degree of crystallinity, while elemental mapping confirmed colocalization of terbium and sulfur. The NPs exhibit characteristic absorbance and luminescence of terbium, with downshifting quantum yield (QY) reaching 28.3% and an emission lifetime of ~ 2 ms.
Conclusions: This high QY and long emission lifetime is unusual in a neat rare-earth compound; it is typically associated with rare-earth ions doped into another crystalline lattice to avoid non-radiative cross relaxation. This suggests a reduced role of nonradiative processes in these terbium-based NPs. This is, to our knowledge, the first report revealing the advantage of biosynthesis over chemical synthesis for Rare Earth Element (REE) based NPs, opening routes to new REE-based nanocrystals.
(© 2024. The Author(s).)
Databáze: MEDLINE
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