Quantification of Pectobacterium carotovorum subsp. carotovorum in kimchi cabbage using a surface-enhanced Raman scattering platform with silver nanostructures.

Autor: Lee SY; Hygienic Safety·Materials Research Group, World Institute of Kimchi, 61755, 86 Kimchi-ro, Nam-gu, Gwangju Metropolitan City, South Korea., Kim SJ; Hygienic Safety·Materials Research Group, World Institute of Kimchi, 61755, 86 Kimchi-ro, Nam-gu, Gwangju Metropolitan City, South Korea., Ha JH; Hygienic Safety·Materials Research Group, World Institute of Kimchi, 61755, 86 Kimchi-ro, Nam-gu, Gwangju Metropolitan City, South Korea. Electronic address: hajee@wikim.re.kr.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2025 Jan 01; Vol. 267, pp. 116766. Date of Electronic Publication: 2024 Sep 07.
DOI: 10.1016/j.bios.2024.116766
Abstrakt: Pectobacterium carotovorum subsp. carotovorum (PCC) is a notorious plant pathogen responsible for severe soft rot in kimchi cabbage, which results in significant economic losses. To detect PCC rapidly and accurately in kimchi cabbage, we developed a surface-enhanced Raman scattering (SERS) substrate on which silver nanospheres (AgNSs), nanowires (AgNWs), and nanoseeds are combined on a polydimethylsiloxane (PDMS) platform. The incorporation of Ag nanoseeds creates a higher density of hotspots, which ensures a low detection limit of 1.001 CFU/mL. Electron microscopy and spectroscopic analyses confirmed the successful fabrication of the substrate and its enhanced sensitivity. The SERS substrate exhibits excellent selectivity by effectively distinguishing PCC from other bacteria commonly found in kimchi cabbage. The substrate gives rise to strong Raman signals across PCC concentrations ranging from 10 1 to 10 6  CFU/mL. Additionally, a predictive model was developed for accurately detecting PCC in real kimchi cabbage samples, and the results were validated by polymerase chain reaction measurements. A sensitive, selective, and rapid approach for PCC detection in kimchi cabbage that offers a promising improvement over existing methodologies is presented.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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