Comparative transcriptome analysis of differentially expressed genes and pathways in male and female flowers of Fraxinus mandshurica.

Autor: Zhang K; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.; College of Life Science, Northeast Forestry University, Harbin, China., Cao Y; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.; College of Life Science, Northeast Forestry University, Harbin, China., Guo X; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.; College of Life Science, Northeast Forestry University, Harbin, China., Kong F; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.; College of Life Science, Northeast Forestry University, Harbin, China., Sun H; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.; College of Life Science, Northeast Forestry University, Harbin, China., Jing T; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.; School of Forestry, Northeast Forestry University, Harbin, China., Zhan Y; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.; College of Life Science, Northeast Forestry University, Harbin, China., Qi F; State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin, China.; College of Life Science, Northeast Forestry University, Harbin, China.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Sep 12; Vol. 19 (9), pp. e0308013. Date of Electronic Publication: 2024 Sep 12 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0308013
Abstrakt: Fraxinus mandshurica Rupr. (F. mandshurica) is a dioecious tree species with important ecological and application values. To delve deeper into the regulatory pathways and genes responsible for male and female flowers in F. mandshurica, we conducted transcriptome sequencing on male and female flowers at four distinct stages. The analysis revealed that the female database generated 38,319,967 reads while the male database generated 43,320,907 reads, resulting in 2930 differentially expressed genes with 1441 were up-regulated and 1489 down-regulated in males compared to females. Following an analysis of Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), four distinct pathways (hormone signal transduction, energy metabolism, flavonoid biosynthesis, and photoperiod) linked to female and male flowers were identified. Subsequently, qRT-PCR verification revealed that FmAUX/IAA, FmEIN3, and FmA-ARR genes in hormone signal transduction pathway are related to female flower development. Meanwhile, FmABF genes in hormone signal transduction pathway, FmGS and FmGDH genes in energy metabolism pathway, FmFLS genes in flavonoid biosynthesis pathway, and FmCaM, FmCRY, and FmPKA genes in photoperiod pathway are related to male flower development. This study was the first to analyze the transcriptome of male and female flowers of F. mandshurica, providing a reference for the developmental pathways and gene expression levels of male and female plants.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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