Salivary Protein Sfapyrase of Spodoptera frugiperda Stimulates Plant Defence Response.

Autor: Yao Y; National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, China., Lin HT; National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, China., Chen YH; National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, China., Chen LL; National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, China., Zhang HL; National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, China., Fu HY; National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, China., Gao SJ; National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, China., Wang R; Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, China., Feng HL; Department of Entomology, Louisiana State University, Baton Rouge, Louisiana, USA., Wang JD; National Engineering Research Center of Sugarcane, Fujian Agriculture and Forestry University, Fuzhou, China.
Jazyk: angličtina
Zdroj: Plant, cell & environment [Plant Cell Environ] 2024 Sep 12. Date of Electronic Publication: 2024 Sep 12.
DOI: 10.1111/pce.15121
Abstrakt: Plants have developed various resistance mechanisms against herbivorous insects through prolonged coevolution. Plant defence responses can be triggered by specific compounds present in insect saliva. Apyrase, a known enzyme that catalyzes the hydrolysis of adenosine triphosphate (ATP) and adenosine diphosphate (ADP) into adenosine monophosphate (AMP) and inorganic phosphorus, has recently been identified in some herbivorous insects. However, whether insect salivary apyrase induces or inhibits plant responses remains poorly understood. In this study, we identified an apyrase-like protein in the salivary proteome of the fall armyworm, Spodoptera frugiperda, named Sfapyrase. Sfapyrase was primarily expressed in the salivary gland and secreted into plants during insect feeding. Transient expression of Sfapyrase in tobacco and maize enhanced plant resistance and resulted in decreased insect feeding. Knockdown of Sfapyrase through RNA interference led to increased growth and feeding of S. frugiperda. Furthermore, we showed that Sfapyrase activates the jasmonic acid signalling pathway and promotes the synthesis of secondary metabolites, especially benzoxazinoids, thereby enhancing resistance to S. frugiperda. In summary, our findings demonstrated that Sfapyrase acts as a salivary elicitor, inducing maize jasmonic acid defence responses and the production of insect-resistant benzoxazinoids. This study provides valuable insights into plant-insect interactions and offers potential targets for developing innovative insect pest management strategies.
(© 2024 John Wiley & Sons Ltd.)
Databáze: MEDLINE
Externí odkaz: