Transcriptomic analysis reveals key molecular signatures across recovery phases of hemorrhagic fever with renal syndrome.

Autor: Hu Y; Medical College, Xijing University, Xi'an, 710199, Shaanxi, People's Republic of China., Wu C; Shapingba Hospital affiliated to Chongqing University (Shapingba District People's Hospital of Chongqing), Chongqing, 400030, People's Republic of China., Li T; Patent Examination Cooperation Sichuan Center of the Patent Office, CNIPA, Chengdu, 610213, Sichuan, People's Republic of China., Wu Y; Xi'an International Medical Center Hospital, Xi'an, 710100, Shaanxi, People's Republic of China., Yao K; Medical College, Xijing University, Xi'an, 710199, Shaanxi, People's Republic of China., Zhang M; Medical College, Xijing University, Xi'an, 710199, Shaanxi, People's Republic of China., Li P; Medical College, Xijing University, Xi'an, 710199, Shaanxi, People's Republic of China., Bian X; School of Public Health, Xi'an Jiaotong University, Xi'an, 710049, Shaanxi, People's Republic of China. bianxuzhao@126.com.
Jazyk: angličtina
Zdroj: BMC medical genomics [BMC Med Genomics] 2024 Sep 11; Vol. 17 (1), pp. 229. Date of Electronic Publication: 2024 Sep 11.
DOI: 10.1186/s12920-024-02004-4
Abstrakt: Background: Hemorrhagic fever with renal syndrome (HFRS), a life-threatening zoonosis caused by hantavirus, poses significant mortality risks and lacks specific treatments. This study aimed to delineate the transcriptomic alterations during the recovery phases of HFRS.
Methods: RNA sequencing was employed to analyze the transcriptomic alterations in peripheral blood mononuclear cells from HFRS patients across the oliguric phase (OP), diuretic phase (DP), and convalescent phase (CP). Twelve differentially expressed genes (DEGs) were validated using quantitative real-time PCR in larger sample sets.
Results: Our analysis revealed pronounced transcriptomic differences between DP and OP, with 38 DEGs showing consistent expression changes across all three phases. Notably, immune checkpoint genes like CD83 and NR4A1 demonstrated a monotonic increase, in contrast to a monotonic decrease observed in antiviral and immunomodulatory genes, including IFI27 and RNASE2. Furthermore, this research elucidates a sustained attenuation of immune responses across three phases, alongside an upregulation of pathways related to tissue repair and regeneration.
Conclusion: Our research reveals the transcriptomic shifts during the recovery phases of HFRS, illuminating key genes and pathways that may serve as biomarkers for disease progression and recovery.
(© 2024. The Author(s).)
Databáze: MEDLINE
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