Inverse blebs operate as hydraulic pumps during mouse blastocyst formation.

Autor: Schliffka MF; Institut Curie, CNRS UMR3215, INSERM U934, PSL Research University and Sorbonne Université, Paris, France.; Carl Zeiss SAS, Marly-le-Roy, France., Dumortier JG; Institut Curie, CNRS UMR3215, INSERM U934, PSL Research University and Sorbonne Université, Paris, France., Pelzer D; Institut Curie, CNRS UMR3215, INSERM U934, PSL Research University and Sorbonne Université, Paris, France., Mukherjee A; Laboratoire de physique de l'École Normale Supérieure, CNRS UMR 8023, PSL Research University, Sorbonne Université and Université Paris Cité, Paris, France. arghyadip.mukherjee@ens.fr., Maître JL; Institut Curie, CNRS UMR3215, INSERM U934, PSL Research University and Sorbonne Université, Paris, France. jean-leon.maitre@curie.fr.
Jazyk: angličtina
Zdroj: Nature cell biology [Nat Cell Biol] 2024 Oct; Vol. 26 (10), pp. 1669-1677. Date of Electronic Publication: 2024 Sep 11.
DOI: 10.1038/s41556-024-01501-z
Abstrakt: During preimplantation development, mouse embryos form a fluid-filled lumen. Pressurized fluid fractures cell-cell contacts and accumulates into pockets, which coarsen into a single lumen. How the embryo controls intercellular fluid movement during coarsening is unknown. Here we report inverse blebs growing into cells at adhesive contacts. Throughout the embryo we observed hundreds of inverse blebs, each filling with intercellular fluid and retracting within a minute. Inverse blebs grow due to pressure build-up resulting from fluid accumulation and cell-cell adhesion, which locally confines fluid. Inverse blebs retract due to actomyosin contraction, practically pushing fluid within the intercellular space. Importantly, inverse blebs occur infrequently at contacts formed by multiple cells, which effectively serve as fluid sinks. Manipulation of the embryo topology reveals that without sinks inverse blebs pump fluid into one another in futile cycles. We propose that inverse blebs operate as hydraulic pumps to promote luminal coarsening, thereby constituting an instrument used by cells to control fluid movement.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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