Novel imaging tools to study mitochondrial morphology in Caenorhabditis elegans .

Autor:	Valera-Alberni M; Department of Molecular Metabolism, Harvard TH Chan School of Public Health, Boston, MA, USA., Yao P; Department of Molecular Metabolism, Harvard TH Chan School of Public Health, Boston, MA, USA., Romero-Sanz S; Department of Molecular Metabolism, Harvard TH Chan School of Public Health, Boston, MA, USA., Lanjuin A; Department of Molecular Metabolism, Harvard TH Chan School of Public Health, Boston, MA, USA., Mair WB; Department of Molecular Metabolism, Harvard TH Chan School of Public Health, Boston, MA, USA wmair@hsph.harvard.edu.
Jazyk:	angličtina
Zdroj:	Life science alliance [Life Sci Alliance] 2024 Sep 11; Vol. 7 (11). Date of Electronic Publication: 2024 Sep 11 (Print Publication: 2024).
DOI:	10.26508/lsa.202402918
Abstrakt:	Mitochondria exhibit a close interplay between their structure and function. Understanding this intricate relationship requires advanced imaging techniques that can capture the dynamic nature of mitochondria and their impact on cellular processes. However, much of the work on mitochondrial dynamics has been performed in single celled organisms or in vitro cell culture. Here, we introduce novel genetic tools for live imaging of mitochondrial morphology in the nematode Caenorhabditis elegans , addressing a pressing need for advanced techniques in studying organelle dynamics within live intact multicellular organisms. Through a comprehensive analysis, we directly compare our tools with existing methods, demonstrating their advantages for visualizing mitochondrial morphology and contrasting their impact on organismal physiology. We reveal limitations of conventional techniques, whereas showcasing the utility and versatility of our approaches, including endogenous CRISPR tags and ectopic labeling. By providing a guide for selecting the most suitable tools based on experimental goals, our work advances mitochondrial research in C. elegans and enhances the strategic integration of diverse imaging modalities for a holistic understanding of organelle dynamics in living organisms. (© 2024 Valera-Alberni et al.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu