Engineering stress as a motivation for filamentous virus morphology.

Autor:	McMahon A; Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, United Kingdom; Kavli Institute for Nanoscience Discovery, Dorothy Crowfoot Hodgkin Building, University of Oxford, Oxford, United Kingdom; Warwick Medical School, University of Warwick, Coventry, United Kingdom. Electronic address: andrew.mcmahon@warwick.ac.uk., Vijayakrishnan S; MRC-University of Glasgow Centre for Virus Research, University of Glasgow, Glasgow, United Kingdom., El Sayyed H; Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, United Kingdom; Kavli Institute for Nanoscience Discovery, Dorothy Crowfoot Hodgkin Building, University of Oxford, Oxford, United Kingdom., Groves D; Warwick Medical School, University of Warwick, Coventry, United Kingdom., Conley MJ; MRC-University of Glasgow Centre for Virus Research, University of Glasgow, Glasgow, United Kingdom., Hutchinson E; MRC-University of Glasgow Centre for Virus Research, University of Glasgow, Glasgow, United Kingdom., Robb NC; Biological Physics Research Group, Clarendon Laboratory, Department of Physics, University of Oxford, Oxford, United Kingdom; Warwick Medical School, University of Warwick, Coventry, United Kingdom. Electronic address: nicole.robb@warwick.ac.uk.
Jazyk:	angličtina
Zdroj:	Biophysical reports [Biophys Rep (N Y)] 2024 Dec 11; Vol. 4 (4), pp. 100181. Date of Electronic Publication: 2024 Sep 10.
DOI:	10.1016/j.bpr.2024.100181
Abstrakt:	Many viruses are pleomorphic in shape and size, with pleomorphism often thought to correlate with infectivity, pathogenicity, or virus survival. For example, influenza and respiratory syncytial virus particles range in size from small spherical virions to filaments reaching many micrometers in length. We have used a pressure vessel model to investigate how the length and width of spherical and filamentous virions can vary for a given critical stress and fluorescence super-resolution microscopy along with image analysis tools to fit imaged influenza viruses to the model. We have shown that influenza virion dimensions fit within the theoretical limits of the model, suggesting that filament formation may be a way to increase an individual virus's volume without particle rupture. We have also used cryoelectron microscopy to investigate influenza and respiratory syncytial virus dimensions at the extrema of the model and used the pressure vessel model to explain the lack of alternative virus particle geometries. Our approach offers insight into the possible purpose of filamentous virus morphology and is applicable to a wide range of other biological entities, including bacteria and fungi. Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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