Metabolomics combined with physiology reveal how white clover (Trifolium repens L.) respond to 6PPD stress.

Autor: Wang JX; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China; Key Laboratory of Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China., Zhang Y; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China; Key Laboratory of Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China., Hu J; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China; Key Laboratory of Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China., Li YF; International Joint Research Center for Persistent Toxic Substances (IJRC-PTS), State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; International Joint Research Center for Arctic Environment and Ecosystem (IJRC-AEE), Polar Academy, Harbin Institute of Technology, Harbin 150090, China., Egorovich KV; Institute of Natural Sciences, M.K. Ammosov North-Eastern Federal University, Yakutsk 677000, Russia., Nikolaevna PN; Institute of Natural Sciences, M.K. Ammosov North-Eastern Federal University, Yakutsk 677000, Russia., Vasilevich MV; Institute of Natural Sciences, M.K. Ammosov North-Eastern Federal University, Yakutsk 677000, Russia., Zhang ZF; International Joint Research Center for Persistent Toxic Substances (IJRC-PTS), State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China; International Joint Research Center for Arctic Environment and Ecosystem (IJRC-AEE), Polar Academy, Harbin Institute of Technology, Harbin 150090, China. Electronic address: zifeng_zhang@aliyun.com., Tang ZH; College of Chemistry, Chemical Engineering and Resource Utilization, Northeast Forestry University, Harbin 150040, China; Key Laboratory of Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin 150040, China. Electronic address: tangzh@nefu.edu.cn.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Dec 01; Vol. 954, pp. 176121. Date of Electronic Publication: 2024 Sep 10.
DOI: 10.1016/j.scitotenv.2024.176121
Abstrakt: As a ubiquitous tire antioxidant, N-(1,3-Dimethyl-butyl)-N'-phenyl-p-phenylene- diamine (6PPD) exists widely in various environmental media and has been detected at high levels in the environment. However, the effects of 6PPD on plants are still poorly understood. In this study, a hydroponic experiment was carried out to investigate the response of white clover (Trifolium repens L.) stressed by 6PPD on physiology and metabolomics. The results indicated that the length of stem and root, as well as biomass were significantly reduced after 500 μg L -1 6PPD treatment. Photosynthetic performances including photosynthetic rate (Pn), stomatal conductance (Gs), intercellular CO 2 concentration (Ci), transpiration rate (Tr) and chlorophyll content of leaves decreased in all treatments except 500 μg L -1 of 6PPD. The malondialdehyde (MDA) content in the shoot of white clover increased by 66.33 % when exposed to 500 μg L -1 of 6PPD compared to control group (CK). Hydrogen peroxide and superoxide anion presented a U-shape trend and began to increase at 500 μg L -1 . Besides, peroxidase and catalase significantly decreased compared to CK after exposure to 500 μg L -1 . Metabolic analysis of clover showed that 6PPD treatment induced changes in 10 metabolic pathways of white clover. Metabolites were significantly down-regulated after exposure to 500 μg L -1 in shoot, while significantly down-regulated in all treatment groups except 500 μg L -1 in root. These findings may provide a novel perspective for phytotoxicity assessment and phytoremediation of 6PPD.
Competing Interests: Declaration of competing interest The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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