Clinical significance of direct fast scarlet staining on the diagnosis of eosinophilic colitis: A comparative study focusing on the eosinophil degranulation in colonic mucosal tissue.

Autor: Ikeda M; Department of Gastroenterology, Fujita Health University Bantane Hospital, Nagoya, Aichi, Japan., Kato H; Department of Gastroenterological Surgery, Fujita Health University School of Medicine Bantane Hospital, Nagoya, Aichi, Japan., Arakawa S; Department of Gastroenterological Surgery, Fujita Health University School of Medicine Bantane Hospital, Nagoya, Aichi, Japan., Kobayashi T; Department of Gastroenterology, Fujita Health University Bantane Hospital, Nagoya, Aichi, Japan., Hashimoto S; Department of Gastroenterology, Fujita Health University Bantane Hospital, Nagoya, Aichi, Japan., Katano Y; Department of Gastroenterology, Fujita Health University Bantane Hospital, Nagoya, Aichi, Japan., Inada KI; Department of Diagnostic Pathology, Fujita Health University, Toyoake, Aichi, Japan., Kiriyama Y; Department of Diagnostic Pathology, Fujita Health University, Toyoake, Aichi, Japan., Ishihara T; Innovative and Clinical Research Promotion Center, Gifu University Hospital, Gifu, Japan., Yamamoto S; Department of Gastroenterology, Fujita Health University Bantane Hospital, Nagoya, Aichi, Japan., Asano Y; Department of Gastroenterological Surgery, Fujita Health University School of Medicine Bantane Hospital, Nagoya, Aichi, Japan., Horiguchi A; Department of Gastroenterological Surgery, Fujita Health University School of Medicine Bantane Hospital, Nagoya, Aichi, Japan.
Jazyk: angličtina
Zdroj: Pathology international [Pathol Int] 2024 Nov; Vol. 74 (11), pp. 632-640. Date of Electronic Publication: 2024 Sep 11.
DOI: 10.1111/pin.13475
Abstrakt: This study aimed to validate the DFS (direct fast scarlet) staining in the diagnosis of EC (eosinophilic colitis). The study included 50 patients with EC and 60 with control colons. Among the 60 control samples, 39 and 21 were collected from the ascending and descending colons, respectively. We compared the median number of eosinophils and frequency of eosinophil degranulation by HE (hematoxylin and eosin) and DFS staining between the EC and control groups. In the right hemi-colon, eosinophil count by HE was useful in distinguishing between EC and control (41.5 vs. 26.0 cells/HPF, p < 0.001), but the ideal cutoff value is 27.5 cells/HPF (high-power field). However, this method is not useful in the left hemi-colon (12.5 vs. 13.0 cells/HPF, p = 0.990). The presence of degranulation by DFS allows us to distinguish between the groups even in the left hemi-colon (58% vs. 5%, p < 0.001). DFS staining also enabled a more accurate determination of degranulation than HE. According to the current standard to diagnose EC (count by HE staining ≥20 cells/HPF), mucosal sampling from left hemi-colon is problematic since the number of eosinophils could not be increased even in EC. Determination of degranulated eosinophils by DFS may potentiate the diagnostic performance even in such conditions.
(© 2024 Japanese Society of Pathology and John Wiley & Sons Australia, Ltd.)
Databáze: MEDLINE
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