| Autor: |
Oliveira BR; Programa de Pós-Gradução em Ciências Fisiológicas, Universidade Federal do Rio Grande/FURG, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular, Av. Itália, Km 8, Campus Carreiros, 96203-900 Rio Grande, RS, Brazil., Marques MB; Programa de Pós-Gradução em Ciências Fisiológicas, Universidade Federal do Rio Grande/FURG, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular, Av. Itália, Km 8, Campus Carreiros, 96203-900 Rio Grande, RS, Brazil., Werhli AV; Universidade Federal do Rio Grande/FURG, Centro de Ciência da Computação (C3), Laboratório de Biologia Computacional, Rodovia, RS-734, s/n, 96203-900 Rio Grande, RS, Brazil., Marins LF; Programa de Pós-Gradução em Ciências Fisiológicas, Universidade Federal do Rio Grande/FURG, Instituto de Ciências Biológicas, Laboratório de Biologia Molecular, Av. Itália, Km 8, Campus Carreiros, 96203-900 Rio Grande, RS, Brazil. |
| Abstrakt: |
We aimed to find new therapeutic targets related to Cancer Stem Cell alterations in recurrent patients from two TCGA cohorts: Testicular Germ Cell Tumor (TGCT) and Uterine Corpus Endometrial Carcinoma (UCEC). Raw sequencing data were downloaded from the TCGA database. Datasets containing RNA expression and Methylation files were directly downloaded from cBioportal. Variant Call Format files (VCFs) were downloaded from the GDC portal. Gene enrichment analysis was performed using GSEA (Gene Set Enrichment Analysis) software. Transcriptome profiling, coexpression co-occurrence, networks, and survival analyses were performed using cBioportal tools, while mutational analysis of patients was processed using UNIX scripts. We found that cancer stem cell transcription factors were highly expressed in Testicular Germ Cell Tumor (TGCT) and Uterine Corpus Endometrial Carcinoma (UCEC) cohorts, compared to the other 29 cancer cohorts in TCGA. Patients presented a poorer diagnosis when the genes (POU5F1, NANOG, SOX2, SALL4, ABCB1, ABCC1, and ABCG2) were altered. In UCEC cohorts, recurrent patients showed the ABCG2 potentially phosphorylated by the PIM1 kinase. In the TGCT cohort, genes ABCB1 and ABCG2 only appeared in the phosphonetwork in recurrent patients potentially phosphorylated by the same kinase, PIM1, but also by PRKACA. Our data indicate that PRKACA and PIM1 may modulate POU5F1 phosphorylation. |
