Hepatic and immune modulatory effectiveness of lactoferrin loaded Selenium nanoparticles on bleomycin induced hepatic injury.

Autor: Abdel-Wahhab KG; Medical Physiology Department, National Research Centre, Giza, 12622, Egypt., Ashry M; Zoology Department, Faculty of Science, Al-Azhar University, Assuit, Egypt., Hassan LK; Dairy Department, National Research Centre, Giza, 12622, Egypt. Lailakhaled1@yahoo.com., El-Azma MH; Medical Physiology Department, National Research Centre, Giza, 12622, Egypt., Elqattan GM; Medical Physiology Department, National Research Centre, Giza, 12622, Egypt., Gadelmawla MHA; Histology Department, Faculty of Dentistry, Sinai University, Kantara, Egypt., Mannaa FA; Medical Physiology Department, National Research Centre, Giza, 12622, Egypt.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Sep 10; Vol. 14 (1), pp. 21066. Date of Electronic Publication: 2024 Sep 10.
DOI: 10.1038/s41598-024-70894-6
Abstrakt: This study aimed to estimate the hepatic and immune ameliorating potential of extracted bovine lactoferrin (LF), Selenium nanoparticles (SeNPs) or their combination (LF/SeNPs) against bleomycin (BLM) induced hepatic injury. Fifty adult male rats (160-200 g) were equally divided into five groups: (1) the saline control group, (2) BLM-injected (15 mg/kg twice a week, ip), and (3-5) groups treated orally with LF (200 mg/kg/day), SeNPs (0.0486 mg/kg/day) or LF/SeNPs combination (200.0486 mg/kg/day) for 6 weeks post BLM-intoxication. Blood and liver samples were subjected to biochemical, histopathological, and immunohistochemical analyses. The results revealed that BLM caused a significant increase in hepatic lipid peroxidation and nitric oxide, as well as serum markers of liver functions (AST, ALT and GGT activities), and levels of GM-CSF, CD4, TNF-α, IL-1β, TGF-β1, fibronectin, triglycerides, cholesterol and LDL-C. Additionally, hepatic glutathione, Na + /K + -ATPase, and glutathione peroxidase, as well as serum HDL-C, total protein and albumin levels were significantly reduced. Moreover, BLM injection resulted in marked histopathological alterations and severe expression of caspase 3. Post-treatment of BLM-intoxicated rats with LF, SeNPs or LF/SeNPs combination obviously improved the BLM-induced hepatic damages; this was achieved from the marked modulations in the mentioned parameters, besides improving the histopathological hepatic architecture. It is worth mentioning that LF/SeNPs exerted the greatest potency. In conclusion, the obtained results demonstrated that LF, SeNPs and LF/SeNPs succeeded in attenuating the BLM-induced hepatic dysfunction. Therefore, these supplements might be used to protect against drug-associated side effects.
(© 2024. The Author(s).)
Databáze: MEDLINE
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