SUB-immunogold-SEM reveals nanoscale distribution of submembranous epitopes.

Autor: Miller KK; Department of Otolaryngology-Head & Neck Surgery, School of Medicine, 240 Pasteur Drive, Stanford University, Stanford, CA, 94305, USA., Wang P; Department of Otolaryngology-Head & Neck Surgery, School of Medicine, 240 Pasteur Drive, Stanford University, Stanford, CA, 94305, USA., Grillet N; Department of Otolaryngology-Head & Neck Surgery, School of Medicine, 240 Pasteur Drive, Stanford University, Stanford, CA, 94305, USA. ngrillet@stanford.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Sep 10; Vol. 15 (1), pp. 7864. Date of Electronic Publication: 2024 Sep 10.
DOI: 10.1038/s41467-024-51849-x
Abstrakt: Electron microscopy paired with immunogold labeling is the most precise tool for protein localization. However, these methods are either cumbersome, resulting in small sample numbers and restricted quantification, or limited to identifying protein epitopes external to the membrane. Here, we introduce SUB-immunogold-SEM, a scanning electron microscopy technique that detects intracellular protein epitopes proximal to the membrane. We identify four critical sample preparation factors contributing to the method's sensitivity. We validate its efficacy through precise localization and high-powered quantification of cytoskeletal and transmembrane protein distribution. We evaluate the capabilities of SUB-immunogold-SEM on cells with highly differentiated apical surfaces: (i) auditory hair cells, revealing the presence of nanoscale MYO15A-L rings at the tip of stereocilia; and (ii) respiratory multiciliate cells, mapping the distribution of the SARS-CoV-2 receptor ACE2 along the motile cilia. SUB-immunogold-SEM extends the application of SEM-based nanoscale protein localization to the detection of intracellular epitopes on the exposed surfaces of any cell.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: