Cu 2 O nanoparticles with morphology-dependent peroxidase mimic activity: a novel colorimetric biosensor for deoxynivalenol detection.

Autor: Zhu X; College of Food Science and Technology, Henan Agricultural University, Zhengzhou, 450002, China., Zhang B; College of Food Science and Technology, Henan Agricultural University, Zhengzhou, 450002, China.; Key Laboratory for Staple Grain Processing, Ministry of Agriculture and Rural Affairs, Zhengzhou, 450002, China., Wang J; College of Food Science and Technology, Henan Agricultural University, Zhengzhou, 450002, China., He Y; College of Food Science and Technology, Henan Agricultural University, Zhengzhou, 450002, China., Chen Z; College of Food Science and Technology, Henan Agricultural University, Zhengzhou, 450002, China., Chang W; Department of Food and Bioengineering, Henan University of Animal Husbandry and Economy, Zhengzhou, 450002, China., Xie X; College of Food Science and Technology, Henan Agricultural University, Zhengzhou, 450002, China. xiexinhua9292@163.com.; Key Laboratory for Staple Grain Processing, Ministry of Agriculture and Rural Affairs, Zhengzhou, 450002, China. xiexinhua9292@163.com., Zhu H; College of Food Science and Technology, Henan Agricultural University, Zhengzhou, 450002, China. zhuhongshuai2021@henau.edu.cn.; Key Laboratory for Staple Grain Processing, Ministry of Agriculture and Rural Affairs, Zhengzhou, 450002, China. zhuhongshuai2021@henau.edu.cn.; Agricultural Engineering Postdoctoral Research Station, Henan Agricultural University, Zhengzhou, 450002, China. zhuhongshuai2021@henau.edu.cn.
Jazyk: angličtina
Zdroj: Mikrochimica acta [Mikrochim Acta] 2024 Sep 10; Vol. 191 (10), pp. 588. Date of Electronic Publication: 2024 Sep 10.
DOI: 10.1007/s00604-024-06676-8
Abstrakt: Different morphological Cu 2 O nanoparticles including cube, truncated cube, and octahedron were successfully prepared by a selective surface stabilization strategy. The prepared cube Cu 2 O exhibited superior peroxidase-like activity over the other two morphological Cu 2 O nanoparticles, which can readily oxidize 3,3',5,5'-tetramethylbenzidine (TMB) to form visually recognizable color signals. Consequently, a sensitive and simple colorimetric biosensor was proposed for deoxynivalenol (DON) detection. In this biosensor, the uniform cube Cu 2 O was employed as the vehicle to label the antibody for the recognition of immunoreaction. The sensing strategy showed a detection limit as low as 0.01 ng/mL, and a wide linear range from 2 to 100 ng/mL. Concurrently, the approximate DON concentration can be immediately and conveniently observed by the vivid color changes. Benefiting from the high sensitivity and selectivity of the designed biosensor, the detection of DON in wheat, corn, and tap water samples was achieved, suggesting the bright prospect of the biosensor for the convenient and intuitive detection of DON in actual samples.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature.)
Databáze: MEDLINE
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