Migration and retention of human osteosarcoma cells in bioceramic graft with open channel architecture designed for bone tissue engineering.

Autor:	G G; Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Science and Technology, Thiruvananthapuram 695012 Kerala, India., Fernandez FB; Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Science and Technology, Thiruvananthapuram 695012 Kerala, India., Varma P R H; Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Science and Technology, Thiruvananthapuram 695012 Kerala, India., Komath M; Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Science and Technology, Thiruvananthapuram 695012 Kerala, India.
Jazyk:	angličtina
Zdroj:	Biomedical materials (Bristol, England) [Biomed Mater] 2024 Sep 18; Vol. 19 (6). Date of Electronic Publication: 2024 Sep 18.
DOI:	10.1088/1748-605X/ad792b
Abstrakt:	The microstructure of a porous bioceramic bone graft, especially the pore architecture, plays a crucial role in the performance of the graft. Conventional bioceramic grafts typically feature a random, closed-pore structure, limiting biological activity to the periphery of the graft. This can lead to delay in full integration with the host site. Bioceramic forms with open through pores can perform better because their inner regions are accessible for natural bone remodeling. This study explores the influence of open through pores in a bioceramic graft on the migration and retention of the local cells in vitro , which will correlate to the rate of healing in vivo. Hydroxyapatite ceramic forms with aligned channels were fabricated using slip casting technique, employing sacrificial fibers. The sorption characteristics across the graft were evaluated using human osteosarcoma cell line. Seven-day cultures showed viable cells within the channels, confirmed by live/dead assay, scanning electron microscope analysis, and cytoskeletal staining, indicating successful cell colonization. The channel architecture effectively enhances cell migration and retention throughout its entire structure, suggesting potential applications in bone tissue engineering based on the results obtained. (© 2024 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu