Arabidopsis hydathodes are sites of auxin accumulation and nutrient scavenging.

Autor: Routaboul JM; Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), Université de Toulouse, INRAE UMR 0441, CNRS UMR 2598, Castanet-Tolosan, F-31326, France., Bellenot C; Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), Université de Toulouse, INRAE UMR 0441, CNRS UMR 2598, Castanet-Tolosan, F-31326, France., Olympio A; Institut de Biosciences et Biotechnologies d'Aix-Marseille, Aix-Marseille Université, CEA, CNRS UMR 7265, Saint Paul-Lez-Durance, F-13108, France., Clément G; Institute Jean-Pierre Bourgin for Plant Sciences (IJPB), Université Paris-Saclay, INRAE, AgroParisTech, Versailles, 78000, France., Citerne S; Institute Jean-Pierre Bourgin for Plant Sciences (IJPB), Université Paris-Saclay, INRAE, AgroParisTech, Versailles, 78000, France., Remblière C; Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), Université de Toulouse, INRAE UMR 0441, CNRS UMR 2598, Castanet-Tolosan, F-31326, France., Charvin M; Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS UMR8197, INSERM U1024, Paris, 75005, France., Franke L; Department of Plant Physiology, Institute for Biology, Martin-Luther-University Halle-Wittenberg, Halle (Saale), D-06120, Germany., Chiarenza S; Institut de Biosciences et Biotechnologies d'Aix-Marseille, Aix-Marseille Université, CEA, CNRS UMR 7265, Saint Paul-Lez-Durance, F-13108, France., Vasselon D; Institute Jean-Pierre Bourgin for Plant Sciences (IJPB), Université Paris-Saclay, INRAE, AgroParisTech, Versailles, 78000, France., Jardinaud MF; Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), Université de Toulouse, INRAE UMR 0441, CNRS UMR 2598, Castanet-Tolosan, F-31326, France., Carrère S; Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), Université de Toulouse, INRAE UMR 0441, CNRS UMR 2598, Castanet-Tolosan, F-31326, France., Nussaume L; Institut de Biosciences et Biotechnologies d'Aix-Marseille, Aix-Marseille Université, CEA, CNRS UMR 7265, Saint Paul-Lez-Durance, F-13108, France., Laufs P; Institute Jean-Pierre Bourgin for Plant Sciences (IJPB), Université Paris-Saclay, INRAE, AgroParisTech, Versailles, 78000, France., Leonhardt N; Institut de Biosciences et Biotechnologies d'Aix-Marseille, Aix-Marseille Université, CEA, CNRS UMR 7265, Saint Paul-Lez-Durance, F-13108, France., Navarro L; Institut de Biologie de l'Ecole Normale Supérieure (IBENS), CNRS UMR8197, INSERM U1024, Paris, 75005, France., Schattat M; Department of Plant Physiology, Institute for Biology, Martin-Luther-University Halle-Wittenberg, Halle (Saale), D-06120, Germany., Noël LD; Laboratoire des Interactions Plantes-Microbes-Environnement (LIPME), Université de Toulouse, INRAE UMR 0441, CNRS UMR 2598, Castanet-Tolosan, F-31326, France.
Jazyk: angličtina
Zdroj: The Plant journal : for cell and molecular biology [Plant J] 2024 Nov; Vol. 120 (3), pp. 857-871. Date of Electronic Publication: 2024 Sep 10.
DOI: 10.1111/tpj.17014
Abstrakt: Hydathodes are small organs found on the leaf margins of vascular plants which release excess xylem sap through a process called guttation. While previous studies have hinted at additional functions of hydathode in metabolite transport or auxin metabolism, experimental support is limited. We conducted comprehensive transcriptomic, metabolomic and physiological analyses of mature Arabidopsis hydathodes. This study identified 1460 genes differentially expressed in hydathodes compared to leaf blades, indicating higher expression of most genes associated with auxin metabolism, metabolite transport, stress response, DNA, RNA or microRNA processes, plant cell wall dynamics and wax metabolism. Notably, we observed differential expression of genes encoding auxin-related transcriptional regulators, biosynthetic processes, transport and vacuolar storage supported by the measured accumulation of free and conjugated auxin in hydathodes. We also showed that 78% of the total content of 52 xylem metabolites was removed from guttation fluid at hydathodes. We demonstrate that NRT2.1 and PHT1;4 transporters capture nitrate and inorganic phosphate in guttation fluid, respectively, thus limiting the loss of nutrients during this process. Our transcriptomic and metabolomic analyses unveil an organ with its specific physiological and biological identity.
(© 2024 Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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