Withaferin A reduces pulmonary eosinophilia and IL-25 production in a mouse model of allergic airways disease.
| Autor: | Agner K; Department of Medicine, Duke University Medical Center, Durham, NC, USA., McQuade VL; Department of Medicine, Duke University Medical Center, Durham, NC, USA., Womble J; Department of Medicine, Duke University Medical Center, Durham, NC, USA., Guttenberg MA; Integrated Toxicology and Environmental Health Program (ITEHP), Duke University, Durham, NC, USA., Phatak S; Diabetes Unit, King Edward Memorial (KEM) Hospital Research Centre, Pune, India., Ingram JL; Department of Medicine, Duke University Medical Center, Durham, NC, USA. |
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| Jazyk: | angličtina |
| Zdroj: | BioRxiv : the preprint server for biology [bioRxiv] 2024 Aug 26. Date of Electronic Publication: 2024 Aug 26. |
| DOI: | 10.1101/2024.08.26.609636 |
| Abstrakt: | Several studies report that ashwagandha, a traditional Ayurvedic supplement, has anti-inflammatory properties. Type 2 (T2) asthma is characterized by eosinophilic airway inflammation. We hypothesized that allergen-induced eosinophilic airway inflammation in mice would be reduced following administration of Withaferin A (WFA), the primary active phytochemical in Ashwagandha. C57BL/6J mice were given 10 total intra-peritoneal injections of 2 mg/kg WFA or vehicle control, concurrent with 6 total intranasal administrations of 50 μg house dust mite extract (HDM) or saline control over 2 weeks. We observed that treatment with WFA reduced allergen-induced peribronchial inflammation and airway eosinophil counts compared to mice treated with controls. In addition, we observed that treatment with WFA reduced lung levels of interleukin-25 (IL-25) but increased lung gene expression levels of its co-receptor, Il17ra , in HDM-challenged mice compared to HDM-challenged mice that received the vehicle control. This study pinpoints a potential mechanism by which WFA modulates allergen-induced airway eosinophilia via the IL-25 signaling pathway. Future studies will investigate the effects of WFA administration on lung eosinophilia and IL-25 signaling in the context of chronic allergen-challenge. Competing Interests: Declaration of Interest Statement The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. |
| Databáze: | MEDLINE |
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