Structural and functional dissection of the Pacinian corpuscle reveals an active role of the inner core in touch detection.

Autor:	Ziolkowski LH; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA., Nikolaev YA; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA., Chikamoto A; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA., Oda M; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA., Feketa VV; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA.; Department of Neuroscience, Yale University School of Medicine, New Haven, CT 06520, USA., Monedero-Alonso D; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA., Ardasheva SA; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA., Bae SS; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA., Xu CS; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA., Pang S; FIB-SEM Collaboration Core, Yale University School of Medicine, New Haven, CT 06520, USA., Gracheva EO; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA.; Department of Neuroscience, Yale University School of Medicine, New Haven, CT 06520, USA.; Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, CT 06520, USA., Bagriantsev SN; Department of Cellular and Molecular Physiology, Yale University School of Medicine, New Haven, CT 06520, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Aug 26. Date of Electronic Publication: 2024 Aug 26.
DOI:	10.1101/2024.08.24.609509
Abstrakt:	Pacinian corpuscles are rapidly adapting mechanoreceptor end-organs that detect transient touch and high-frequency vibration. In the prevailing model, these properties are determined by the outer core, which acts as a mechanical filter limiting static and low-frequency stimuli from reaching the afferent terminal-the sole site of touch detection in corpuscles. Here, we determine the detailed 3D architecture of corpuscular components and reveal their contribution to touch detection. We show that the outer core is dispensable for rapid adaptation and frequency tuning. Instead, these properties arise from the inner core, composed of gap junction-coupled lamellar Schwann cells (LSCs) surrounding the afferent terminal. By acting as additional touch sensing structures, LSCs potentiate mechanosensitivity of the terminal, which detects touch via fast-inactivating ion channels. We propose a model in which Pacinian corpuscle function is mediated by an interplay between mechanosensitive LSCs and the afferent terminal in the inner core. Competing Interests: Competing Interests. CSX is an inventor of a US patent assigned to Howard Hughes Medical Institute for the enhanced FIB-SEM systems used in this work: Xu, C.S., Hayworth K.J., Hess H.F. (2020) Enhanced FIB-SEM systems for large-volume 3D imaging. US Patent 10,600,615, 24 Mar 2020. The authors declare no other competing interests.
Databáze:	MEDLINE
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