Ubiquitin-driven protein condensation stabilizes clathrin-mediated endocytosis.

Autor:	Yuan F; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA., Gollapudi S; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA., Day KJ; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA., Ashby G; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA., Sangani A; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA., Malady BT; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA., Wang L; Department of Biochemistry and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA., Lafer EM; Department of Biochemistry and Structural Biology, The University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA., Huibregtse JM; Department of Molecular Biosciences, The University of Texas at Austin, Austin, TX 78712, USA., Stachowiak JC; Department of Biomedical Engineering, The University of Texas at Austin, Austin, TX 78712, USA.; McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712, USA.
Jazyk:	angličtina
Zdroj:	PNAS nexus [PNAS Nexus] 2024 Aug 21; Vol. 3 (9), pp. pgae342. Date of Electronic Publication: 2024 Aug 21 (Print Publication: 2024).
DOI:	10.1093/pnasnexus/pgae342
Abstrakt:	Clathrin-mediated endocytosis is an essential cellular pathway that enables signaling and recycling of transmembrane proteins and lipids. During endocytosis, dozens of cytosolic proteins come together at the plasma membrane, assembling into a highly interconnected network that drives endocytic vesicle biogenesis. Recently, multiple groups have reported that early endocytic proteins form flexible condensates, which provide a platform for efficient assembly of endocytic vesicles. Given the importance of this network in the dynamics of endocytosis, how might cells regulate its stability? Many receptors and endocytic proteins are ubiquitylated, while early endocytic proteins such as Eps15 contain ubiquitin-interacting motifs. Therefore, we examined the influence of ubiquitin on the stability of the early endocytic protein network. In vitro, we found that recruitment of small amounts of polyubiquitin dramatically increased the stability of Eps15 condensates, suggesting that ubiquitylation could nucleate endocytic assemblies. In live-cell imaging experiments, a version of Eps15 that lacked the ubiquitin-interacting motif failed to rescue defects in endocytic initiation created by Eps15 knockout. Furthermore, fusion of Eps15 to a deubiquitylase enzyme destabilized nascent endocytic sites within minutes. In both in vitro and live-cell settings, dynamic exchange of Eps15 proteins, a measure of protein network stability, was decreased by Eps15-ubiquitin interactions and increased by loss of ubiquitin. These results collectively suggest that ubiquitylation drives assembly of the flexible protein network responsible for catalyzing endocytic events. More broadly, this work illustrates a biophysical mechanism by which ubiquitylated transmembrane proteins at the plasma membrane could regulate the efficiency of endocytic internalization. (© The Author(s) 2024. Published by Oxford University Press on behalf of National Academy of Sciences.)
Databáze:	MEDLINE
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