Lysosomes accumulate at the perinuclear region of muscle cells during chick myogenesis.

Autor: Bagri KM; Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Pereira MG; Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de janeiro, Rio de Janeiro, Brazil., Leichtweis KS; Laboratório de Embriologia de Vertebrados, Instituto de Ciências Biomédicas, Universidade Federal do Rio de janeiro, Rio de Janeiro, Brazil., Abreu JG; Laboratório de Embriologia de Vertebrados, Instituto de Ciências Biomédicas, Universidade Federal do Rio de janeiro, Rio de Janeiro, Brazil., Costa ML; Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Mermelstein C; Instituto de Ciências Biomédicas, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Jazyk: angličtina
Zdroj: Cell biology international [Cell Biol Int] 2024 Nov; Vol. 48 (11), pp. 1625-1636. Date of Electronic Publication: 2024 Sep 09.
DOI: 10.1002/cbin.12238
Abstrakt: Lysosomes are involved in a myriad of cellular functions, such as degradation of macromolecules, endocytosis and exocytosis, modulation of several signaling pathways, and regulation of cell metabolism. To fulfill these diverse functions, lysosomes can undergo several dynamic changes in their content, size, pH, and location within cells. Here, we studied some of these parameters during embryonic chick skeletal muscle cells. We used an anti-lysosome-associated membrane protein 2 (LAMP2) antibody to specifically determine the intracellular localization of lysosomes in these cells. Our data shows that lysosomes are highly enriched in the perinuclear region of chick embryonic muscle cells. We also showed that the wingless signaling pathway (Wnt)/β-catenin signaling pathway can modulate the location of LAMP2 in chick myogenic cells. Our results highlight the role of lysosomes during muscle differentiation and particularly the presence of a subcellular population of lysosomes that are concentrated in the perinuclear region of muscle cells.
(© 2024 International Federation for Cell Biology.)
Databáze: MEDLINE