Application of the luminous bacterium Photobacterium phosphoreum for toxicity monitoring of selenite and its reduction to selenium(0) nanoparticles.

Autor: Zenkov AV; School of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia., Sushko ES; School of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036 Krasnoyarsk, Russia; Institute of Physics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036 Krasnoyarsk, Russia. Electronic address: kkovel@yandex.ru., Mogilnaya OA; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036 Krasnoyarsk, Russia., Volochaev MN; Institute of Physics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036 Krasnoyarsk, Russia., Shabanov AV; Institute of Physics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036 Krasnoyarsk, Russia., Kamnev AA; Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Federal Scientific Center of the Russian Academy of Sciences, 410049 Saratov, Russia., Tugarova AV; Institute of Biochemistry and Physiology of Plants and Microorganisms, Saratov Federal Scientific Center of the Russian Academy of Sciences, 410049 Saratov, Russia., Kudryasheva NS; School of Fundamental Biology and Biotechnology, Siberian Federal University, 660041 Krasnoyarsk, Russia; Institute of Biophysics SB RAS, Federal Research Center 'Krasnoyarsk Science Center SB RAS', 660036 Krasnoyarsk, Russia. Electronic address: n-qdr@yandex.ru.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2025 Jan 15; Vol. 325, pp. 125078. Date of Electronic Publication: 2024 Sep 01.
DOI: 10.1016/j.saa.2024.125078
Abstrakt: Luminous marine bacteria are traditionally used as a bioassay due to the convenience and high rate of registering the intensity of their physiological function - luminescence. This study aimed to develop the application of Photobacterium phosphoreum in traditional and novel fields - toxicity monitoring and biotechnology. We demonstrated (1) effects of selenite ions on bioluminescence, and (2) biotransformation of selenite to selenium(0) in the form of nanoparticles. The effects of selenite (SeO 3 2- ) on the intensity of bacterial bioluminescence were studied, and its dependencies on exposure time and concentration of Na 2 SeO 3 were analyzed. Bioluminescence activation and inhibition were revealed; dose-effect dependencies corresponded to the hormesis model. The toxicity of SeO 3 2- was characterized by an effective concentration of 10 -3 M. Effects of SeO 3 2- on reactive oxygen species (ROS) in bacterial suspensions were studied. High positive correlations were found between the bioluminescence intensity and ROS content, which indicates the decisive role of ROS and associated redox processes in the bioeffects of selenite ions. Scanning and transmission electron microscopy revealed the presence of nano-structures in the bacteria exposed to selenite. The energy dispersion spectrum detected a high content of selenium in the nanoparticles. The particle size distribution depended on Na 2 SeO 3 concentration; maxima of the distribution varied within 45-55 nm.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Nadezhda S. Kudryasheva reports financial support was provided by Russian Science Foundation and Krasnoyarsk Territory and Krasnoyarsk Regional Fund of Science. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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