DcMYB62, a transcription factor from carrot, enhanced cadmium tolerance of Arabidopsis by inducing the accumulation of carotenoids and hydrogen sulfide.

Autor: Sun M; College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng, Jiangsu 224002, China; State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China., Qiao HX; College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng, Jiangsu 224002, China., Yang T; College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng, Jiangsu 224002, China., Zhao P; College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng, Jiangsu 224002, China., Zhao JH; College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng, Jiangsu 224002, China., Luo JM; College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng, Jiangsu 224002, China., Liu FF; College of Marine and Biological Engineering, Yancheng Teachers University, Yancheng, Jiangsu 224002, China., Xiong AS; State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China. Electronic address: xiongaisheng@njau.edu.cn.
Jazyk: angličtina
Zdroj: Plant physiology and biochemistry : PPB [Plant Physiol Biochem] 2024 Nov; Vol. 216, pp. 109114. Date of Electronic Publication: 2024 Sep 07.
DOI: 10.1016/j.plaphy.2024.109114
Abstrakt: Cadmium (Cd) is a significant heavy metal contaminant within the environment, carrying a notable level of toxicity that presents a substantial hazard to both plant and human. Carrot (Daucus carota), a significant root vegetable crop globally, have evolved multiple transcriptional regulatory mechanisms to cope with Cd stress, with a crucial involvement of the myeloblastosis (MYB) transcription factor. In this study, the DcMYB62 gene encoding 288 amino acids, localized in the nucleus and demonstrated transcription activation property, was isolated from carrot (cv. 'Kuroda'). There was a positive relationship observed between the levels of DcMYB62 expression and the accumulation patterns of carotenoids in two distinct carrot cultivars. Further investigation revealed that the expression of DcMYB62 improved Cd tolerance of Arabidopsis by increasing seed germination rate, root length, and overall survival rate. The levels of carotenoids in DcMYB62 transgenic Arabidopsis surpassed those in wild type, accompanied by elevated expression levels of 15-cis-phytoene desaturase, zeta-carotene desaturase, and carotenoid isomerase. Meanwhile, the heterologous expression of DcMYB62 promoted the biosynthesis of abscisic acid (ABA) and hydrogen sulfide (H 2 S), which in turn suppressed the formation of hydrogen peroxide and superoxide anion, while also stimulating stomatal closure. Furthermore, the heterologous expression of DcMYB62 increased the transcription of genes associated with heavy metal resistance in Arabidopsis, notably nicotianamine synthase. Overall, this study contributes to understanding how DcMYB62 promote Cd stress resistance of plants by regulating the biosynthesis pathways of carotenoids, ABA, and H 2 S, which offers valuable insights into the regulatory mechanism connecting DcMYBs with Cd stress response of carrot.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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