Association of MTR and MTRR polymorphisms with recurrent pregnancy loss: a case control study.

Autor: Shaker MM; Prenatal Diagnosis and Fetal Medicine Department, Human Genetics and Genome Research Institute, NationalResearch Centre, Cairo, Egypt. maishaker777@gmail.com., Elaraby NM; Medical Molecular Genetics Department, Human Genetics and Genome Research Institute, National ResearchCentre, Cairo, Egypt., Shalabi TA; Prenatal Diagnosis and Fetal Medicine Department, Human Genetics and Genome Research Institute, NationalResearch Centre, Cairo, Egypt.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2024 Sep 09; Vol. 51 (1), pp. 971. Date of Electronic Publication: 2024 Sep 09.
DOI: 10.1007/s11033-024-09860-4
Abstrakt: Background: In light of several epidemiological studies, the etiology of recurrent pregnancy loss is complex. One of the most frequent causes of women experiencing inexplicable recurrent pregnancy loss is maternal thrombophilia. Hence, the association between genetic polymorphisms causing thrombophilia and recurrent pregnancy loss needs to be explored.
Aim: Is to study the relation of polymorphisms affecting folate pathway mainly, 5-Methytetrahydrofolate-Homocysteine Methyltransferase (MTR A2756G) and 5-Methytetrahydrofolate-Homocysteine MethyltransferaseReductase (MTRR A66G) with recurrent pregnancy loss.
Methods: It is a case-control study. Four hundred participants were enrolled. Two hundred participants with unexplained recurrent pregnancy loss (case group) and two hundred healthy fertile participants (control group). All participants were screened for (MTR A2756G) and (MTRR A66G). DNA was extracted using salting out method followed by genotyping via Real-time PCR.
Results: Mutant homozygous genotype (GG) in MTRR A66G was statistically significantly among RPL group in comparison to controls. (GG vs. AA) had odds ratio and confidence interval of 1.22(1.12-2.23), P = 0.012. (GG) increased the liability 1.2 folds for recurrent pregnancy loss. Mutant homozygous genotype (GG) in MTR A2756G was not correlated with the risk of recurrent pregnancy loss. (GG vs.AA) = (1.13(0.56-2.29)), P = 0.7 CONCLUSION: MTRR A66G increases susceptibly for recurrent pregnancy loss among Egyptian women.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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