Peripheral Serotonin Controls Dietary Fat Absorption and Chylomicron Secretion via 5-HT4 Receptor in Males.

Autor:	Raka F; Department of Physiology, University of Toronto, Toronto, ON M5S 1A8, Canada.; Molecular Medicine, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada., Hoffman S; Molecular Medicine, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada., Nady A; Molecular Medicine, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada., Guan H; Molecular Medicine, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada., Zhang R; Molecular Medicine, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada., Wang H; Department of Pathology & Molecular Medicine and Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON L8S 4K1, Canada., Khan WI; Department of Pathology & Molecular Medicine and Farncombe Family Digestive Health Research Institute, McMaster University, Hamilton, ON L8S 4K1, Canada., Adeli K; Department of Physiology, University of Toronto, Toronto, ON M5S 1A8, Canada.; Molecular Medicine, Research Institute, The Hospital for Sick Children, Toronto, ON M5G 0A4, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON M5S 1A8, Canada.
Jazyk:	angličtina
Zdroj:	Endocrinology [Endocrinology] 2024 Aug 27; Vol. 165 (10).
DOI:	10.1210/endocr/bqae112
Abstrakt:	Postprandial dyslipidemia is commonly present in people with type 2 diabetes and obesity and is characterized by overproduction of apolipoprotein B48-containing chylomicron particles from the intestine. Peripheral serotonin is emerging as a regulator of energy homeostasis with profound implications for obesity; however, its role in dietary fat absorption and chylomicron production is unknown. Chylomicron production was assessed in Syrian golden hamsters by administering an olive oil gavage and IP poloxamer to inhibit lipoprotein clearance. Administration of serotonin or selective serotonin reuptake inhibitor, fluoxetine, increased postprandial plasma triglyceride (TG) and TG-rich lipoproteins. Conversely, inhibiting serotonin synthesis pharmacologically by p-chlorophenylalanine (PCPA) led to a reduction in both the size and number of TG-rich lipoprotein particles, resulting in lower plasma TG and apolipoprotein B48 levels. The effects of PCPA occurred independently of gastric emptying and vagal afferent signaling. Inhibiting serotonin synthesis by PCPA led to increased TG within the intestinal lumen and elevated levels of TG and cholesterol in the stool when exposed to a high-fat/high-cholesterol diet. These findings imply compromised fat absorption, as evidenced by reduced lipase activity in the duodenum and lower levels of serum bile acids, which are indicative of intestinal bile acids. During the postprandial state, mRNA levels for serotonin receptors (5-HTRs) were upregulated in the proximal intestine. Administration of cisapride, a 5-HT4 receptor agonist, alleviated reductions in postprandial lipemia caused by serotonin synthesis inhibition, indicating that serotonin controls dietary fat absorption and chylomicron secretion via 5-HT4 receptor. (© The Author(s) 2024. Published by Oxford University Press on behalf of the Endocrine Society. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com. See the journal About page for additional terms.)
Databáze:	MEDLINE
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