Eosinophils respond to, but are not essential for control of an acute Salmonella enterica serovar Typhimurium infection in mice.

Autor:	FitzPatrick RD; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada., Noone JR; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada., Cartwright RA; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada., Gatti DM; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada., Brosschot TP; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada., Lane JM; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada., Jensen EL; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada., Kroker Kimber I; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada., Reynolds LA; Department of Biochemistry and Microbiology, University of Victoria, Victoria, British Columbia, Canada.
Jazyk:	angličtina
Zdroj:	Infection and immunity [Infect Immun] 2024 Oct 15; Vol. 92 (10), pp. e0032524. Date of Electronic Publication: 2024 Sep 09.
DOI:	10.1128/iai.00325-24
Abstrakt:	Eosinophils are a highly abundant cell type in the gastrointestinal tract during homeostatic conditions, where they have recently been reported to take on an activated phenotype following colonization by the bacterial microbiota. To date, there have been few studies investigating whether eosinophils respond to infection with enteric bacterial pathogens and/or investigating the requirements for eosinophils for effective bacterial pathogen control. In this study, we investigated the response of eosinophils to an acute enteric infection of mice with the bacterial pathogen Salmonella enterica serovar Typhimurium. We also assessed whether eosinophil deficiency impacted Salmonella burdens in the intestinal tract or impacted the systemic dissemination of Salmonella following an oral infection of littermate wild-type BALB/cJ and eosinophil-deficient ΔdblGATA BALB/cJ mice. We found comparable Salmonella burdens in the intestinal tract of wild-type and eosinophil-deficient mice and no significant differences in the levels of Salmonella disseminating to systemic organs within 3 days of infection. Despite our evidence suggesting that eosinophils are not an essential cell type for controlling bacterial burdens in this acute infection setting, we found higher levels of eosinophils in gut-draining lymph nodes following infection, indicating that eosinophils do respond to Salmonella infection. Our data contribute to the growing evidence that eosinophils are responsive to bacterial stimuli, yet the influence of and requirements for eosinophils during bacterial infection appear to be highly context-dependent. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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